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Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells
LC. Noack, P. Pejchar, J. Sekereš, Y. Jaillais, M. Potocký,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Gene Expression MeSH
- Fluorescent Dyes analysis metabolism MeSH
- Microscopy, Fluorescence methods MeSH
- Phosphatidylinositols analysis metabolism MeSH
- Phospholipids analysis metabolism MeSH
- Microscopy, Confocal methods MeSH
- Luminescent Proteins analysis genetics MeSH
- Pollen chemistry genetics MeSH
- Plant Cells chemistry metabolism MeSH
- Nicotiana chemistry cytology genetics MeSH
- Transformation, Genetic MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Anionic phospholipids represent only minor fraction of cell membranes lipids but they are critically important for many membrane-related processes, including membrane identity, charge, shape, the generation of second messengers, and the recruitment of peripheral proteins. The main anionic phospholipids of the plasma membrane are phosphoinositides phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 4,5-bisphosphate (PI4,5P2), phosphatidylserine (PS), and phosphatidic acid (PA). Recent insights in the understanding of the nature of protein-phospholipid interactions enabled the design of genetically encoded fluorescent molecular probes that can interact with various phospholipids in a specific manner allowing their imaging in live cells. Here, we describe the use of transiently transformed plant cells to study phospholipid-dependent membrane recruitment.
References provided by Crossref.org
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