Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Microscopy, Nicotiana benthamiana, Nicotiana tabacum, Phosphoinositides, Phospholipid-binding domains, Pollen tube, Transient expression,
- MeSH
- Gene Expression MeSH
- Fluorescent Dyes analysis metabolism MeSH
- Microscopy, Fluorescence methods MeSH
- Phosphatidylinositols analysis metabolism MeSH
- Phospholipids analysis metabolism MeSH
- Microscopy, Confocal methods MeSH
- Luminescent Proteins analysis genetics MeSH
- Pollen chemistry genetics MeSH
- Plant Cells chemistry metabolism MeSH
- Nicotiana chemistry cytology genetics MeSH
- Transformation, Genetic MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Fluorescent Dyes MeSH
- Phosphatidylinositols MeSH
- Phospholipids MeSH
- Luminescent Proteins MeSH
Anionic phospholipids represent only minor fraction of cell membranes lipids but they are critically important for many membrane-related processes, including membrane identity, charge, shape, the generation of second messengers, and the recruitment of peripheral proteins. The main anionic phospholipids of the plasma membrane are phosphoinositides phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 4,5-bisphosphate (PI4,5P2), phosphatidylserine (PS), and phosphatidic acid (PA). Recent insights in the understanding of the nature of protein-phospholipid interactions enabled the design of genetically encoded fluorescent molecular probes that can interact with various phospholipids in a specific manner allowing their imaging in live cells. Here, we describe the use of transiently transformed plant cells to study phospholipid-dependent membrane recruitment.
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
Institute of Experimental Botany Czech Academy of Sciences Prague Czech Republic
References provided by Crossref.org
A protocol for in vivo RNA labeling and visualization in tobacco pollen tubes
Exploring lipid-protein interactions in plant membranes
Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants
