Transient Gene Expression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
- Klíčová slova
- Microscopy, Nicotiana benthamiana, Nicotiana tabacum, Phosphoinositides, Phospholipid-binding domains, Pollen tube, Transient expression,
- MeSH
- exprese genu MeSH
- fluorescenční barviva analýza metabolismus MeSH
- fluorescenční mikroskopie metody MeSH
- fosfatidylinositoly analýza metabolismus MeSH
- fosfolipidy analýza metabolismus MeSH
- konfokální mikroskopie metody MeSH
- luminescentní proteiny analýza genetika MeSH
- pyl chemie genetika MeSH
- rostlinné buňky chemie metabolismus MeSH
- tabák chemie cytologie genetika MeSH
- transformace genetická MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fluorescenční barviva MeSH
- fosfatidylinositoly MeSH
- fosfolipidy MeSH
- luminescentní proteiny MeSH
Anionic phospholipids represent only minor fraction of cell membranes lipids but they are critically important for many membrane-related processes, including membrane identity, charge, shape, the generation of second messengers, and the recruitment of peripheral proteins. The main anionic phospholipids of the plasma membrane are phosphoinositides phosphatidylinositol 4-phosphate (PI4P), phosphatidylinositol 4,5-bisphosphate (PI4,5P2), phosphatidylserine (PS), and phosphatidic acid (PA). Recent insights in the understanding of the nature of protein-phospholipid interactions enabled the design of genetically encoded fluorescent molecular probes that can interact with various phospholipids in a specific manner allowing their imaging in live cells. Here, we describe the use of transiently transformed plant cells to study phospholipid-dependent membrane recruitment.
Department of Experimental Plant Biology Faculty of Science Charles University Prague Czech Republic
Institute of Experimental Botany Czech Academy of Sciences Prague Czech Republic
Citace poskytuje Crossref.org
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