-
Je něco špatně v tomto záznamu ?
Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions
A. Jelínková, K. Malínská, J. Petrášek,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- Arabidopsis cytologie ultrastruktura MeSH
- buněčná membrána ultrastruktura MeSH
- endocytóza * MeSH
- fluorescenční barviva analýza MeSH
- fluorescenční mikroskopie metody MeSH
- kořeny rostlin cytologie ultrastruktura MeSH
- optické zobrazování metody MeSH
- tabák cytologie ultrastruktura MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
FM (Fei-Mao) styryl dyes are commonly used for the fluorescence imaging of plasma membrane (PM) and endocytosis in vivo. Thanks to their amphiphilic character, these dyes are incorporated in the outer leaflet of the PM lipid bilayer and emit fluorescence in its hydrophobic environment. The endocytic pathway of FM dye uptake starts with rapid PM staining and continues in PM invaginations and membrane vesicles during endocytosis, followed by staining of trans-Golgi network (TGN) and ending in tonoplast (vacuolar membrane). FM dyes do not stain endoplasmic reticulum and nuclear membrane. The time-lapse fluorescence microscopy could track endocytic vesicles and characterize the rate of endocytosis in vivo. On the other hand, fixable FM dyes (FX) can be used for the visualization of particular steps in the FM dye uptake in situ. Staining with FM dyes and subsequent microscopic observations could be performed on both tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and destaining in root tip of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20006311
- 003
- CZ-PrNML
- 005
- 20200518132532.0
- 007
- ta
- 008
- 200511s2019 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/978-1-4939-9469-4_11 $2 doi
- 035 __
- $a (PubMed)31148038
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Jelínková, Adriana $u Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic. jelinkovaa@ueb.cas.cz.
- 245 10
- $a Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions / $c A. Jelínková, K. Malínská, J. Petrášek,
- 520 9_
- $a FM (Fei-Mao) styryl dyes are commonly used for the fluorescence imaging of plasma membrane (PM) and endocytosis in vivo. Thanks to their amphiphilic character, these dyes are incorporated in the outer leaflet of the PM lipid bilayer and emit fluorescence in its hydrophobic environment. The endocytic pathway of FM dye uptake starts with rapid PM staining and continues in PM invaginations and membrane vesicles during endocytosis, followed by staining of trans-Golgi network (TGN) and ending in tonoplast (vacuolar membrane). FM dyes do not stain endoplasmic reticulum and nuclear membrane. The time-lapse fluorescence microscopy could track endocytic vesicles and characterize the rate of endocytosis in vivo. On the other hand, fixable FM dyes (FX) can be used for the visualization of particular steps in the FM dye uptake in situ. Staining with FM dyes and subsequent microscopic observations could be performed on both tissue and cellular level. Here, we describe simple procedures for the effective FM dye staining and destaining in root tip of Arabidopsis thaliana seedlings and suspension-cultured tobacco cells.
- 650 _2
- $a Arabidopsis $x cytologie $x ultrastruktura $7 D017360
- 650 _2
- $a buněčná membrána $x ultrastruktura $7 D002462
- 650 12
- $a endocytóza $7 D004705
- 650 _2
- $a fluorescenční barviva $x analýza $7 D005456
- 650 _2
- $a fluorescenční mikroskopie $x metody $7 D008856
- 650 _2
- $a optické zobrazování $x metody $7 D061848
- 650 _2
- $a kořeny rostlin $x cytologie $x ultrastruktura $7 D018517
- 650 _2
- $a tabák $x cytologie $x ultrastruktura $7 D014026
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Malínská, Kateřina $u Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.
- 700 1_
- $a Petrášek, Jan $u Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic. Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.
- 773 0_
- $w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 1992, č. - (2019), s. 173-187
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/31148038 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20200511 $b ABA008
- 991 __
- $a 20200518132531 $b ABA008
- 999 __
- $a ok $b bmc $g 1525169 $s 1096367
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2019 $b 1992 $c - $d 173-187 $e - $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
- LZP __
- $a Pubmed-20200511
