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Multicomponent Yeast Two-Hybrid System: Applications to Study Protein-Protein Interactions in SMC Complexes
JJ. Paleček, L. Vondrová, K. Zábrady, J. Otočka,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- chromozomy hub fyziologie MeSH
- mapy interakcí proteinů fyziologie MeSH
- multiproteinové komplexy metabolismus MeSH
- podjednotky proteinů metabolismus MeSH
- proteiny buněčného cyklu metabolismus MeSH
- Saccharomyces cerevisiae - proteiny metabolismus MeSH
- Saccharomyces cerevisiae metabolismus MeSH
- techniky dvojhybridového systému MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Analysis of protein-protein interactions (PPI) is key for the understanding of most protein assemblies including structural maintenance of chromosomes (SMC) complexes. SMC complexes are composed of SMC proteins, kleisin, and kleisin-interacting subunits. These subunits interact in specific ways to constitute and regulate the closed structure of the complexes. Specifically, kleisin molecules bridge the SMC dimers and the kleisin-interacting subunits modulate stability of the bridge. Here we describe a multicomponent version of a yeast two-hybrid (Y2H) method and its application for analysis of the bridging role of the Nse4 kleisin in the SMC5/6 complex. Using this technique, we also show a stabilizing effect of KITE (kleisin-interacting tandem winged-helix element) proteins on SMC5/6.
Genome Damage and Stability Centre University of Sussex Brighton UK
National Centre for Biomolecular Research Faculty of Science Masaryk University Brno Czech Republic
Citace poskytuje Crossref.org
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- $a Paleček, Jan Josef $u National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic. jpalecek@sci.muni.cz. Mendel Centre for Plant Genomics and Proteomics, Central European Institute of Technology, Masaryk University, Brno, Czech Republic. jpalecek@sci.muni.cz.
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