Despite great efforts, the design of antibacterial surfaces is still a challenge. In this work, results of structural, mechanical, cytotoxic and antibacterial activities of hydrophobic carbon quantum dots/polydimethylsiloxane surfaces are presented. Antibacterial action of this surface is based on the generation of reactive oxygen species which cause bacteria damage by oxidative stress. At the same time, this surface was not cytotoxic towards the NIH/3T3 cells. Swelling-encapsulation-shrink method is applied for encapsulation of hydrophobic carbon quantum dots in medical grade silicone-polydimethylsiloxane. XPS and photoluminescence spectroscopy analyses confirm that hydrophobic carbon quantum dots have been encapsulated successfully into polydimethylsiloxane polymer matrix. Based on stress-strain test the improvement of mechanical properties of these nanocomposites is established. It is shown by electron paramagnetic resonance spectroscopy and luminescence method that nanocomposite generates singlet oxygen initiated by 470 nm blue light irradiation. Antibacterial testing shows the nanocomposite in the form of foil kills Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and is very effective after only a 15 min irradiation.

Centre of Polymer Systems Tomas Bata University in Zlín Trida Tomase Bati 5678 Zlín Czech Republic

J Heyrovský Institute of Physical Chemistry Academy of Sciences of the Czech Republic Dolejškova 3 182 23 Praha 8 Czech Republic

Polymer Institute Slovak Academy of Sciences Dúbravská cesta 9 84541 Bratislava Slovakia

Vinča Institute of Nuclear Sciences University of Belgrade P O B 522 11001 Belgrade Serbia

000      

00000naa a2200000 a 4500

001      

bmc20006425

003      

CZ-PrNML

005      

20200526152029.0

007      

ta

008      

200511s2019 ne f 000 0|eng||

009      

AR

024    7_

$a 10.1016/j.pdpdt.2019.04.019 $2 doi

035    __

$a (PubMed)31022579

040    __

$a ABA008 $b cze $d ABA008 $e AACR2

041    0_

$a eng

044    __

$a ne

100    1_

$a Marković, Zoran M $u Vinča Institute of Nuclear Sciences, University of Belgrade, P.O.B. 522, 11001 Belgrade, Serbia. Electronic address: zoranmarkovic@vin.bg.ac.rs.

245    10

$a Antibacterial photodynamic activity of carbon quantum dots/polydimethylsiloxane nanocomposites against Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae / $c ZM. Marković, M. Kováčová, P. Humpolíček, MD. Budimir, J. Vajďák, P. Kubát, M. Mičušík, H. Švajdlenková, M. Danko, Z. Capáková, M. Lehocký, BM. Todorović Marković, Z. Špitalský,

520    9_

$a Despite great efforts, the design of antibacterial surfaces is still a challenge. In this work, results of structural, mechanical, cytotoxic and antibacterial activities of hydrophobic carbon quantum dots/polydimethylsiloxane surfaces are presented. Antibacterial action of this surface is based on the generation of reactive oxygen species which cause bacteria damage by oxidative stress. At the same time, this surface was not cytotoxic towards the NIH/3T3 cells. Swelling-encapsulation-shrink method is applied for encapsulation of hydrophobic carbon quantum dots in medical grade silicone-polydimethylsiloxane. XPS and photoluminescence spectroscopy analyses confirm that hydrophobic carbon quantum dots have been encapsulated successfully into polydimethylsiloxane polymer matrix. Based on stress-strain test the improvement of mechanical properties of these nanocomposites is established. It is shown by electron paramagnetic resonance spectroscopy and luminescence method that nanocomposite generates singlet oxygen initiated by 470 nm blue light irradiation. Antibacterial testing shows the nanocomposite in the form of foil kills Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and is very effective after only a 15 min irradiation.

650    _2

$a zvířata $7 D000818

650    _2

$a uhlík $x farmakologie $7 D002244

650    _2

$a dimethylpolysiloxany $x farmakologie $7 D004129

650    _2

$a Escherichia coli $x účinky léků $7 D004926

650    _2

$a Klebsiella pneumoniae $x účinky léků $7 D007711

650    _2

$a myši $7 D051379

650    _2

$a nanokompozity $x terapeutické užití $7 D053761

650    _2

$a fotochemoterapie $x metody $7 D010778

650    _2

$a kvantové tečky $x terapeutické užití $7 D045663

650    _2

$a singletový kyslík $x metabolismus $7 D026082

650    _2

$a Staphylococcus aureus $x účinky léků $7 D013211

650    _2

$a povrchové vlastnosti $7 D013499

655    _2

$a časopisecké články $7 D016428

700    1_

$a Kováčová, Mária $u Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, Slovakia.

700    1_

$a Humpolíček, Petr $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, Zlín, Czech Republic.

700    1_

$a Budimir, Milica D $u Vinča Institute of Nuclear Sciences, University of Belgrade, P.O.B. 522, 11001 Belgrade, Serbia.

700    1_

$a Vajďák, Jan $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, Zlín, Czech Republic.

700    1_

$a Kubát, Pavel $u J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Dolejškova 3, 182 23 Praha 8, Czech Republic.

700    1_

$a Mičušík, Matej $u Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, Slovakia.

700    1_

$a Švajdlenková, Helena $u Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, Slovakia.

700    1_

$a Danko, Martin $u Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, Slovakia.

700    1_

$a Capáková, Zdenka $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, Zlín, Czech Republic.

700    1_

$a Lehocký, Marián $u Centre of Polymer Systems, Tomas Bata University in Zlín, Trida Tomase Bati 5678, Zlín, Czech Republic.

700    1_

$a Todorović Marković, Biljana M $u Vinča Institute of Nuclear Sciences, University of Belgrade, P.O.B. 522, 11001 Belgrade, Serbia.

700    1_

$a Špitalský, Zdeno $u Polymer Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 84541 Bratislava, Slovakia. Electronic address: zdeno.spitalsky@savba.sk.

773    0_

$w MED00007528 $t Photodiagnosis and photodynamic therapy $x 1873-1597 $g Roč. 26, č. - (2019), s. 342-349

856    41

$u https://pubmed.ncbi.nlm.nih.gov/31022579 $y Pubmed

910    __

$a ABA008 $b sig $c sign $y a $z 0

990    __

$a 20200511 $b ABA008

991    __

$a 20200526152026 $b ABA008

999    __

$a ok $b bmc $g 1525283 $s 1096481

BAS    __

$a 3

BAS    __

$a PreBMC

BMC    __

$a 2019 $b 26 $c - $d 342-349 $e 20190422 $i 1873-1597 $m Photodiagnosis and photodynamic therapy $n Photodiagn Photodyn Ther $x MED00007528

LZP    __

$a Pubmed-20200511