This study presents a toxicological evaluation of two types of carbon dots (CD), similar in size (<10 nm) but differing in surface charge. Whole-genome mRNA and miRNA expression (RNAseq), as well as gene-specific DNA methylation changes, were analyzed in human embryonic lung fibroblasts (HEL 12469) after 4 h and 24 h exposure to concentrations of 10 and 50 µg/mL (for positive charged CD; pCD) or 10 and 100 µg/mL (for negative charged CD, nCD). The results showed a distinct response for the tested nanomaterials (NMs). The exposure to pCD induced the expression of a substantially lower number of mRNAs than those to nCD, with few commonly differentially expressed genes between the two CDs. For both CDs, the number of deregulated mRNAs increased with the dose and exposure time. The pathway analysis revealed a deregulation of processes associated with immune response, tumorigenesis and cell cycle regulation, after exposure to pCD. For nCD treatment, pathways relating to cell proliferation, apoptosis, oxidative stress, gene expression, and cycle regulation were detected. The expression of miRNAs followed a similar pattern: more pronounced changes after nCD exposure and few commonly differentially expressed miRNAs between the two CDs. For both CDs the pathway analysis based on miRNA-mRNA interactions, showed a deregulation of cancer-related pathways, immune processes and processes involved in extracellular matrix interactions. DNA methylation was not affected by exposure to any of the two CDs. In summary, although the tested CDs induced distinct responses on the level of mRNA and miRNA expression, pathway analyses revealed a potential common biological impact of both NMs independent of their surface charge.
- MeSH
- apoptóza účinky léků genetika MeSH
- exprese genu účinky léků genetika MeSH
- extracelulární matrix genetika MeSH
- fibroblasty účinky léků MeSH
- kultivované buňky MeSH
- lidé MeSH
- messenger RNA genetika MeSH
- metylace DNA účinky léků genetika MeSH
- mikro RNA genetika MeSH
- nádory genetika MeSH
- oxidační stres účinky léků genetika MeSH
- plíce účinky léků MeSH
- proliferace buněk účinky léků genetika MeSH
- signální transdukce účinky léků genetika MeSH
- stanovení celkové genové exprese metody MeSH
- uhlík farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Despite great efforts, the design of antibacterial surfaces is still a challenge. In this work, results of structural, mechanical, cytotoxic and antibacterial activities of hydrophobic carbon quantum dots/polydimethylsiloxane surfaces are presented. Antibacterial action of this surface is based on the generation of reactive oxygen species which cause bacteria damage by oxidative stress. At the same time, this surface was not cytotoxic towards the NIH/3T3 cells. Swelling-encapsulation-shrink method is applied for encapsulation of hydrophobic carbon quantum dots in medical grade silicone-polydimethylsiloxane. XPS and photoluminescence spectroscopy analyses confirm that hydrophobic carbon quantum dots have been encapsulated successfully into polydimethylsiloxane polymer matrix. Based on stress-strain test the improvement of mechanical properties of these nanocomposites is established. It is shown by electron paramagnetic resonance spectroscopy and luminescence method that nanocomposite generates singlet oxygen initiated by 470 nm blue light irradiation. Antibacterial testing shows the nanocomposite in the form of foil kills Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and is very effective after only a 15 min irradiation.
- MeSH
- dimethylpolysiloxany farmakologie MeSH
- Escherichia coli účinky léků MeSH
- fotochemoterapie metody MeSH
- Klebsiella pneumoniae účinky léků MeSH
- kvantové tečky terapeutické užití MeSH
- myši MeSH
- nanokompozity terapeutické užití MeSH
- povrchové vlastnosti MeSH
- singletový kyslík metabolismus MeSH
- Staphylococcus aureus účinky léků MeSH
- uhlík farmakologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- MeSH
- DNA vazebné proteiny fyziologie genetika metabolismus MeSH
- izoenzymy genetika MeSH
- mitochondriální ADP/ATP-translokasy genetika MeSH
- regulace genové exprese u hub účinky léků MeSH
- responzivní elementy genetika MeSH
- Saccharomyces cerevisiae enzymologie genetika účinky léků MeSH
- uhlík farmakologie MeSH
39 s. ; 20 cm
- MeSH
- uhlík farmakologie farmakokinetika terapeutické užití MeSH
- Publikační typ
- vysokoškolské kvalifikační práce MeSH
- Konspekt
- Farmacie. Farmakologie
- NLK Obory
- farmacie a farmakologie