Despite great efforts, the design of antibacterial surfaces is still a challenge. In this work, results of structural, mechanical, cytotoxic and antibacterial activities of hydrophobic carbon quantum dots/polydimethylsiloxane surfaces are presented. Antibacterial action of this surface is based on the generation of reactive oxygen species which cause bacteria damage by oxidative stress. At the same time, this surface was not cytotoxic towards the NIH/3T3 cells. Swelling-encapsulation-shrink method is applied for encapsulation of hydrophobic carbon quantum dots in medical grade silicone-polydimethylsiloxane. XPS and photoluminescence spectroscopy analyses confirm that hydrophobic carbon quantum dots have been encapsulated successfully into polydimethylsiloxane polymer matrix. Based on stress-strain test the improvement of mechanical properties of these nanocomposites is established. It is shown by electron paramagnetic resonance spectroscopy and luminescence method that nanocomposite generates singlet oxygen initiated by 470 nm blue light irradiation. Antibacterial testing shows the nanocomposite in the form of foil kills Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae and is very effective after only a 15 min irradiation.
- MeSH
- dimethylpolysiloxany farmakologie MeSH
- Escherichia coli účinky léků MeSH
- fotochemoterapie metody MeSH
- Klebsiella pneumoniae účinky léků MeSH
- kvantové tečky terapeutické užití MeSH
- myši MeSH
- nanokompozity terapeutické užití MeSH
- povrchové vlastnosti MeSH
- singletový kyslík metabolismus MeSH
- Staphylococcus aureus účinky léků MeSH
- uhlík farmakologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
A deep understanding of the interaction between cancerous cells and surfaces is particularly important for the design of lab-on-chip devices involving the use of polydimethylsiloxane (PDMS). In our studies, the effect of PDMS substrate stiffness on mechanical properties of cancerous cells was investigated in conditions where the PDMS substrate is not covered with any of extracellular matrix proteins. Two human prostate cancer (Du145 and PC-3) and two melanoma (WM115 and WM266-4) cell lines were cultured on two groups of PDMS substrates that were characterized by distinct stiffness, i.e. 0.75 ± 0.06 MPa and 2.92 ± 0.12 MPa. The results showed the strong effect on cellular behavior and morphology. The detailed analysis of chemical and physical properties of substrates revealed that cellular behavior occurs only due to substrate elasticity.
- MeSH
- biomechanika MeSH
- dimethylpolysiloxany chemie farmakologie MeSH
- fibrinogen chemie MeSH
- lidé MeSH
- mechanické jevy * MeSH
- melanom patologie MeSH
- mikročipové analytické postupy MeSH
- nádorové buněčné linie MeSH
- nádory prostaty patologie MeSH
- povrchové vlastnosti MeSH
- proliferace buněk účinky léků MeSH
- pružnost MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- MeSH
- dimethylpolysiloxany farmakologie MeSH
- flatulence farmakoterapie MeSH
- lidé MeSH
- Check Tag
- lidé MeSH
