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Molecular characterization of divergent grapevine Pinot gris virus isolates and their detection in Slovak and Czech grapevines
M. Glasa, L. Predajňa, P. Komínek, A. Nagyová, T. Candresse, A. Olmos,
Language English Country Austria
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
ProQuest Central
from 2002-01-01 to 1 year ago
Medline Complete (EBSCOhost)
from 2000-01-01 to 1 year ago
Health & Medicine (ProQuest)
from 2002-01-01 to 1 year ago
Public Health Database (ProQuest)
from 2002-01-01 to 1 year ago
- MeSH
- Flexiviridae classification genetics isolation & purification MeSH
- Phylogeny MeSH
- Genetic Variation * MeSH
- Genome, Viral MeSH
- Molecular Sequence Data MeSH
- Plant Diseases virology MeSH
- Open Reading Frames MeSH
- Vitis virology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
- Slovakia MeSH
Analysis of complete genome sequences of three Slovak isolates of grapevine Pinot gris virus (GPGV) showed their low heterogeneity (reaching 1.7 %) and a close relationship to the Italian NC_015782 isolate (4.2-4.5 % divergence). Comparison of Slovak and Italian isolates revealed an unusual accumulation of 21 indel mutations in ORF1, resulting in a localized high divergence in the encoded amino acid sequences. An elevated divergence in the 5' extremity of the GPGV genomes is suggestive of a recombination between Slovak isolates and grapevine berry inner necrosis virus. RT-PCR allowed the frequent detection of closely related GPGV isolates in grapevines from Slovakia and the Czech Republic.
References provided by Crossref.org
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