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Grapevine virus T is relatively widespread in Slovakia and Czech Republic and genetically diverse

M. Glasa, L. Predajňa, N. Sihelská, K. Šoltys, AB. Ruiz-García, A. Olmos, T. Wetzel, S. Sabanadzovic,

. 2018 ; 54 (5) : 737-741. [pub] 20180711

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc20015897

Grantová podpora
VEGA 2/0036/16 Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
APVV-15-0232 Agentúra na Podporu Výskumu a Vývoja
ITMS313021D075 European rural development fund
FA1407 European Cooperation in Science and Technology

E-zdroje Online Plný text

NLK ProQuest Central od 1997-01-01 do Před 1 rokem
Medline Complete (EBSCOhost) od 2011-02-01 do Před 1 rokem
Health & Medicine (ProQuest) od 1997-01-01 do Před 1 rokem
Public Health Database (ProQuest) od 1997-01-01 do Před 1 rokem

A recently described putative foveavirus, grapevine virus T (GVT), was detected in a Slovak grapevine accession (SK704) using high-throughput sequencing, prompting further studies. Full-length genome sequence of isolate GVT-SK704 was determined. Analyses revealed 86.1% nucleotide identity with the Italian GVT isolate, currently the only available nearly complete sequence of GVT in GenBank. A virus-specific RT-PCR assay was developed, which enabled a survey of GVT incidence in grapevine samples from Slovakia and Czech Republic. Unexpectedly, GVT was present in ~ 30% of tested samples. Analysis of complete CP gene sequences of 20 Slovak and Czech GVT isolates detected in the survey revealed relatively high intra-species variability (up to 11.2% nucleotide divergence), suggesting multiple introductions from different sources, possibly over an extended period of time.

Citace poskytuje Crossref.org

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