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Limited diagnostic possibilities for bloodstream infections with broad-range methods: A promising PCR/electrospray ionization-mass spectrometry platform is no longer available

Jan Tkadlec, Eliska Bebrova, Jan Berousek, Tomas Vymazal, Jaroslava Adamkova, Vendula Martinkova, Claus Moser, Dragos Florea, Pavel Drevinek

. 2020 ; 9 (5) : e1007.

Language English Country Great Britain

Document type Research Support, Non-U.S. Gov't, Clinical Study

Persistent link   https://www.medvik.cz/link/bmc20016477

Grant support
NV15-28157A MZ0 CEP Register

Fast and accurate detection of causative agents of bloodstream infections remains a challenge of today's microbiology. We compared the performance of cutting-edge technology based on polymerase chain reaction coupled with electrospray ionization-mass spectrometry (PCR/ESI-MS) with that of conventional broad-range 16S rRNA PCR and blood culture to address the current diagnostic possibilities for bloodstream infections. Of 160 blood samples tested, PCR/ESI-MS revealed clinically meaningful microbiological agents in 47 samples that were missed by conventional diagnostic approaches (29.4% of all analyzed samples). Notably, PCR/ESI-MS shortened the time to positivity of the blood culture-positive samples by an average of 34 hr. PCR/ESI-MS technology substantially improved current diagnostic tools and represented an opportunity to make bloodstream infections diagnostics sensitive, accurate, and timely with a broad spectrum of microorganisms covered.

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$a Bébrová, Eliška $7 xx0107803 $u Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czech Republic
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$a Beroušek, Jan $7 xx0240692 $u Department of Anaesthesiology and Intensive Care Medicine, 2nd Faculty of Medicine, Charles University and Motol University Hospital, Prague, Czech Republic
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