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Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

CW. Hiebert, MJ. Moscou, T. Hewitt, B. Steuernagel, I. Hernández-Pinzón, P. Green, V. Pujol, P. Zhang, MN. Rouse, Y. Jin, RA. McIntosh, N. Upadhyaya, J. Zhang, S. Bhavani, J. Vrána, M. Karafiátová, L. Huang, T. Fetch, J. Doležel, BBH. Wulff, E....

. 2020 ; 11 (1) : 1123. [pub] 20200228

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.

Perzistentní odkaz   https://www.medvik.cz/link/bmc20023154

Grantová podpora
IOS-0965429 National Science Foundation (NSF) - International
BB/P012574/1 Biotechnology and Biological Sciences Research Council - United Kingdom
BB/P016855/1 Biotechnology and Biological Sciences Research Council - United Kingdom

Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.

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$a Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.
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$a Moscou, Matthew J $u The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, NR4 7UK, UK. matthew.moscou@tsl.ac.uk.
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$a Hewitt, Tim $u Plant Breeding Institute Cobbitty, University of Sydney, Private Bag 4011, Narellan, NSW, 2567, Australia. CSIRO Agriculture & Food, GPO Box 1700, Canberra, ACT, 2601, Australia.
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$a Steuernagel, Burkhard $u John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
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$a Hernández-Pinzón, Inma $u The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, NR4 7UK, UK.
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$a Pujol, Vincent $u Research School of Biology, The Australian National University, Acton, ACT, 2601, Australia.
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$a Zhang, Peng $u Plant Breeding Institute Cobbitty, University of Sydney, Private Bag 4011, Narellan, NSW, 2567, Australia.
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$a Rouse, Matthew N $u USDA-ARS, Cereal Disease Laboratory, University of Minnesota, St. Paul, MN, 55108, USA.
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$a Jin, Yue $u USDA-ARS, Cereal Disease Laboratory, University of Minnesota, St. Paul, MN, 55108, USA.
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$a McIntosh, Robert A $u Plant Breeding Institute Cobbitty, University of Sydney, Private Bag 4011, Narellan, NSW, 2567, Australia.
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$a Upadhyaya, Narayana $u CSIRO Agriculture & Food, GPO Box 1700, Canberra, ACT, 2601, Australia.
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$a Zhang, Jianping $u CSIRO Agriculture & Food, GPO Box 1700, Canberra, ACT, 2601, Australia.
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$a Bhavani, Sridhar $u CIMMYT, ICRAF House, United Nations Avenue, Gigiri, Village Market, Nairobi, 00621, Kenya.
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$a Vrána, Jan $u Institute of Experimental Botany, Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 779 00, Olomouc, Czech Republic.
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$a Karafiátová, Miroslava $u Institute of Experimental Botany, Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 779 00, Olomouc, Czech Republic.
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$a Huang, Li $u Department of Plant Sciences and Plant Pathology, Montana State University, Bozeman, MT, 59717, USA.
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$a Fetch, Tom $u Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada.
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$a Doležel, Jaroslav $u Institute of Experimental Botany, Czech Academy of Sciences, Centre of the Region Haná for Biotechnological and Agricultural Research, Šlechtitelů 31, 779 00, Olomouc, Czech Republic.
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$a Wulff, Brande B H $u John Innes Centre, Norwich Research Park, Norwich, NR4 7UH, UK.
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$a Lagudah, Evans $u CSIRO Agriculture & Food, GPO Box 1700, Canberra, ACT, 2601, Australia. Evans.Lagudah@csiro.au.
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$a Spielmeyer, Wolfgang $u CSIRO Agriculture & Food, GPO Box 1700, Canberra, ACT, 2601, Australia. Wolfgang.Spielmeyer@csiro.au.
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