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Stem rust resistance in wheat is suppressed by a subunit of the mediator complex
CW. Hiebert, MJ. Moscou, T. Hewitt, B. Steuernagel, I. Hernández-Pinzón, P. Green, V. Pujol, P. Zhang, MN. Rouse, Y. Jin, RA. McIntosh, N. Upadhyaya, J. Zhang, S. Bhavani, J. Vrána, M. Karafiátová, L. Huang, T. Fetch, J. Doležel, BBH. Wulff, E....
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
Grantová podpora
IOS-0965429
National Science Foundation (NSF) - International
BB/P012574/1
Biotechnology and Biological Sciences Research Council - United Kingdom
BB/P016855/1
Biotechnology and Biological Sciences Research Council - United Kingdom
NLK
Directory of Open Access Journals
od 2015
Free Medical Journals
od 2010
Nature Open Access
od 2010-12-01
PubMed Central
od 2012
Europe PubMed Central
od 2012
ProQuest Central
od 2010-01-01
Open Access Digital Library
od 2015-01-01
Open Access Digital Library
od 2015-01-01
Medline Complete (EBSCOhost)
od 2012-11-01
Health & Medicine (ProQuest)
od 2010-01-01
ROAD: Directory of Open Access Scholarly Resources
od 2010
- MeSH
- Basidiomycota patogenita MeSH
- chromozomy rostlin genetika MeSH
- duplikace genu MeSH
- exprese genu MeSH
- fenotyp MeSH
- imunita rostlin genetika MeSH
- lipnicovité klasifikace genetika MeSH
- mapování chromozomů MeSH
- mediátorový komplex genetika MeSH
- mutace MeSH
- nemoci rostlin genetika imunologie mikrobiologie MeSH
- odolnost vůči nemocem genetika MeSH
- pšenice genetika imunologie mikrobiologie MeSH
- regulace genové exprese u rostlin MeSH
- rostlinné geny genetika MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.
CIMMYT ICRAF House United Nations Avenue Gigiri Village Market Nairobi 00621 Kenya
CSIRO Agriculture and Food GPO Box 1700 Canberra ACT 2601 Australia
Department of Plant Sciences and Plant Pathology Montana State University Bozeman MT 59717 USA
John Innes Centre Norwich Research Park Norwich NR4 7UH UK
Plant Breeding Institute Cobbitty University of Sydney Private Bag 4011 Narellan NSW 2567 Australia
Research School of Biology The Australian National University Acton ACT 2601 Australia
The Sainsbury Laboratory University of East Anglia Norwich Research Park Norwich NR4 7UK UK
USDA ARS Cereal Disease Laboratory University of Minnesota St Paul MN 55108 USA
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- $a Hiebert, Colin W $u Agriculture and Agri-Food Canada, Morden Research and Development Centre, 101 Route 100, Morden, MB, R6M 1Y5, Canada. Colin.Hiebert@AGR.GC.CA.
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- $a Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24 h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat.
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