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Distinct nitrogen cycling and steep chemical gradients in Trichodesmium colonies
I. Klawonn, MJ. Eichner, ST. Wilson, N. Moradi, B. Thamdrup, S. Kümmel, M. Gehre, A. Khalili, HP. Grossart, DM. Karl, H. Ploug,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
Europe PubMed Central od 2011 do Před 1 rokem
ProQuest Central od 2007-05-01 do Před 1 rokem
Health & Medicine (ProQuest) od 2007-05-01 do Před 1 rokem
Odkazy
PubMed
31636364
DOI
10.1038/s41396-019-0514-9
Knihovny.cz E-zdroje
- MeSH
- amoniové sloučeniny metabolismus MeSH
- autotrofní procesy MeSH
- denitrifikace MeSH
- dusičnany metabolismus MeSH
- dusík metabolismus MeSH
- fixace dusíku MeSH
- koloběh dusíku MeSH
- koloběh uhlíku MeSH
- kyslík metabolismus MeSH
- mořská voda mikrobiologie MeSH
- nitrifikace MeSH
- oxid uhličitý metabolismus MeSH
- Trichodesmium metabolismus MeSH
- uhlík metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
Trichodesmium is an important dinitrogen (N2)-fixing cyanobacterium in marine ecosystems. Recent nucleic acid analyses indicate that Trichodesmium colonies with their diverse epibionts support various nitrogen (N) transformations beyond N2 fixation. However, rates of these transformations and concentration gradients of N compounds in Trichodesmium colonies remain largely unresolved. We combined isotope-tracer incubations, micro-profiling and numeric modelling to explore carbon fixation, N cycling processes as well as oxygen, ammonium and nitrate concentration gradients in individual field-sampled Trichodesmium colonies. Colonies were net-autotrophic, with carbon and N2 fixation occurring mostly during the day. Ten percent of the fixed N was released as ammonium after 12-h incubations. Nitrification was not detectable but nitrate consumption was high when nitrate was added. The consumed nitrate was partly reduced to ammonium, while denitrification was insignificant. Thus, the potential N transformation network was characterised by fixed N gain and recycling processes rather than denitrification. Oxygen concentrations within colonies were ~60-200% air-saturation. Moreover, our modelling predicted steep concentration gradients, with up to 6-fold higher ammonium concentrations, and nitrate depletion in the colony centre compared to the ambient seawater. These gradients created a chemically heterogeneous microenvironment, presumably facilitating diverse microbial metabolisms in millimetre-sized Trichodesmium colonies.
Department of Isotope Biogeochemistry Helmholtz Centre for Environmental Research Leipzig Germany
Department of Marine Sciences University of Gothenburg Gothenburg Sweden
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- $a Klawonn, Isabell $u Department of Ecology, Environment and Plant Sciences, Stockholm University, Stockholm, Sweden. klawonn@stanford.edu. Department of Experimental Limnology, IGB-Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany. klawonn@stanford.edu. Department of Earth System Science, Stanford University, Stanford, CA, USA. klawonn@stanford.edu.
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- $a Distinct nitrogen cycling and steep chemical gradients in Trichodesmium colonies / $c I. Klawonn, MJ. Eichner, ST. Wilson, N. Moradi, B. Thamdrup, S. Kümmel, M. Gehre, A. Khalili, HP. Grossart, DM. Karl, H. Ploug,
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- $a Trichodesmium is an important dinitrogen (N2)-fixing cyanobacterium in marine ecosystems. Recent nucleic acid analyses indicate that Trichodesmium colonies with their diverse epibionts support various nitrogen (N) transformations beyond N2 fixation. However, rates of these transformations and concentration gradients of N compounds in Trichodesmium colonies remain largely unresolved. We combined isotope-tracer incubations, micro-profiling and numeric modelling to explore carbon fixation, N cycling processes as well as oxygen, ammonium and nitrate concentration gradients in individual field-sampled Trichodesmium colonies. Colonies were net-autotrophic, with carbon and N2 fixation occurring mostly during the day. Ten percent of the fixed N was released as ammonium after 12-h incubations. Nitrification was not detectable but nitrate consumption was high when nitrate was added. The consumed nitrate was partly reduced to ammonium, while denitrification was insignificant. Thus, the potential N transformation network was characterised by fixed N gain and recycling processes rather than denitrification. Oxygen concentrations within colonies were ~60-200% air-saturation. Moreover, our modelling predicted steep concentration gradients, with up to 6-fold higher ammonium concentrations, and nitrate depletion in the colony centre compared to the ambient seawater. These gradients created a chemically heterogeneous microenvironment, presumably facilitating diverse microbial metabolisms in millimetre-sized Trichodesmium colonies.
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- $a Eichner, Meri J $u Department of Marine Sciences, University of Gothenburg, Gothenburg, Sweden. Centre A lgatech, Institute of Microbiology, The Czech Academy of Sciences, Trebon, Czech Republic.
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