-
Je něco špatně v tomto záznamu ?
Anaerobic peroxisomes in Mastigamoeba balamuthi
T. Le, V. Žárský, E. Nývltová, P. Rada, K. Harant, M. Vancová, Z. Verner, I. Hrdý, J. Tachezy,
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 1915 do Před 6 měsíci
Freely Accessible Science Journals
od 1915 do Před 6 měsíci
PubMed Central
od 1915 do Před 6 měsíci
Europe PubMed Central
od 1915 do Před 6 měsíci
Open Access Digital Library
od 1915-01-15
Open Access Digital Library
od 1915-01-01
PubMed
31932444
DOI
10.1073/pnas.1909755117
Knihovny.cz E-zdroje
- MeSH
- anaerobióza MeSH
- Archamoebae genetika metabolismus MeSH
- mitochondrie genetika metabolismus MeSH
- oxidace-redukce MeSH
- peroxiny genetika metabolismus MeSH
- peroxizomy genetika metabolismus MeSH
- protozoální proteiny genetika metabolismus MeSH
- reaktivní formy kyslíku metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The adaptation of eukaryotic cells to anaerobic conditions is reflected by substantial changes to mitochondrial metabolism and functional reduction. Hydrogenosomes belong among the most modified mitochondrial derivative and generate molecular hydrogen concomitant with ATP synthesis. The reduction of mitochondria is frequently associated with loss of peroxisomes, which compartmentalize pathways that generate reactive oxygen species (ROS) and thus protect against cellular damage. The biogenesis and function of peroxisomes are tightly coupled with mitochondria. These organelles share fission machinery components, oxidative metabolism pathways, ROS scavenging activities, and some metabolites. The loss of peroxisomes in eukaryotes with reduced mitochondria is thus not unexpected. Surprisingly, we identified peroxisomes in the anaerobic, hydrogenosome-bearing protist Mastigamoeba balamuthi We found a conserved set of peroxin (Pex) proteins that are required for protein import, peroxisomal growth, and division. Key membrane-associated Pexs (MbPex3, MbPex11, and MbPex14) were visualized in numerous vesicles distinct from hydrogenosomes, the endoplasmic reticulum (ER), and Golgi complex. Proteomic analysis of cellular fractions and prediction of peroxisomal targeting signals (PTS1/PTS2) identified 51 putative peroxisomal matrix proteins. Expression of selected proteins in Saccharomyces cerevisiae revealed specific targeting to peroxisomes. The matrix proteins identified included components of acyl-CoA and carbohydrate metabolism and pyrimidine and CoA biosynthesis, whereas no components related to either β-oxidation or catalase were present. In conclusion, we identified a subclass of peroxisomes, named "anaerobic" peroxisomes that shift the current paradigm and turn attention to the reductive evolution of peroxisomes in anaerobic organisms.
Citace poskytuje Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20023221
- 003
- CZ-PrNML
- 005
- 20201214125522.0
- 007
- ta
- 008
- 201125s2020 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1073/pnas.1909755117 $2 doi
- 035 __
- $a (PubMed)31932444
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Le, Tien $u Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 25242 Vestec, Czech Republic.
- 245 10
- $a Anaerobic peroxisomes in Mastigamoeba balamuthi / $c T. Le, V. Žárský, E. Nývltová, P. Rada, K. Harant, M. Vancová, Z. Verner, I. Hrdý, J. Tachezy,
- 520 9_
- $a The adaptation of eukaryotic cells to anaerobic conditions is reflected by substantial changes to mitochondrial metabolism and functional reduction. Hydrogenosomes belong among the most modified mitochondrial derivative and generate molecular hydrogen concomitant with ATP synthesis. The reduction of mitochondria is frequently associated with loss of peroxisomes, which compartmentalize pathways that generate reactive oxygen species (ROS) and thus protect against cellular damage. The biogenesis and function of peroxisomes are tightly coupled with mitochondria. These organelles share fission machinery components, oxidative metabolism pathways, ROS scavenging activities, and some metabolites. The loss of peroxisomes in eukaryotes with reduced mitochondria is thus not unexpected. Surprisingly, we identified peroxisomes in the anaerobic, hydrogenosome-bearing protist Mastigamoeba balamuthi We found a conserved set of peroxin (Pex) proteins that are required for protein import, peroxisomal growth, and division. Key membrane-associated Pexs (MbPex3, MbPex11, and MbPex14) were visualized in numerous vesicles distinct from hydrogenosomes, the endoplasmic reticulum (ER), and Golgi complex. Proteomic analysis of cellular fractions and prediction of peroxisomal targeting signals (PTS1/PTS2) identified 51 putative peroxisomal matrix proteins. Expression of selected proteins in Saccharomyces cerevisiae revealed specific targeting to peroxisomes. The matrix proteins identified included components of acyl-CoA and carbohydrate metabolism and pyrimidine and CoA biosynthesis, whereas no components related to either β-oxidation or catalase were present. In conclusion, we identified a subclass of peroxisomes, named "anaerobic" peroxisomes that shift the current paradigm and turn attention to the reductive evolution of peroxisomes in anaerobic organisms.
- 650 _2
- $a anaerobióza $7 D000693
- 650 _2
- $a Archamoebae $x genetika $x metabolismus $7 D056896
- 650 _2
- $a mitochondrie $x genetika $x metabolismus $7 D008928
- 650 _2
- $a oxidace-redukce $7 D010084
- 650 _2
- $a peroxiny $x genetika $x metabolismus $7 D000074401
- 650 _2
- $a peroxizomy $x genetika $x metabolismus $7 D020675
- 650 _2
- $a protozoální proteiny $x genetika $x metabolismus $7 D015800
- 650 _2
- $a reaktivní formy kyslíku $x metabolismus $7 D017382
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Žárský, Vojtěch $u Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 25242 Vestec, Czech Republic.
- 700 1_
- $a Nývltová, Eva $u Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 25242 Vestec, Czech Republic.
- 700 1_
- $a Rada, Petr $u Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 25242 Vestec, Czech Republic.
- 700 1_
- $a Harant, Karel $u Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 25242 Vestec, Czech Republic.
- 700 1_
- $a Vancová, Marie $u Institute of Parasitology, Biology Centre, Czech Academy of Sciences, 370 05 České Budějovice, Czech Republic.
- 700 1_
- $a Verner, Zdeněk $u Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 25242 Vestec, Czech Republic.
- 700 1_
- $a Hrdý, Ivan $u Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 25242 Vestec, Czech Republic.
- 700 1_
- $a Tachezy, Jan $u Department of Parasitology, Faculty of Science, BIOCEV, Charles University, 25242 Vestec, Czech Republic; tachezy@natur.cuni.cz.
- 773 0_
- $w MED00010472 $t Proceedings of the National Academy of Sciences of the United States of America $x 1091-6490 $g Roč. 117, č. 4 (2020), s. 2065-2075
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/31932444 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20201125 $b ABA008
- 991 __
- $a 20201214125521 $b ABA008
- 999 __
- $a ok $b bmc $g 1595540 $s 1113897
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 117 $c 4 $d 2065-2075 $e 20200113 $i 1091-6490 $m Proceedings of the National Academy of Sciences of the United States of America $n Proc Natl Acad Sci U S A $x MED00010472
- LZP __
- $a Pubmed-20201125
