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Trans-splicing of mRNAs links gene transcription to translational control regulated by mTOR

GB. Danks, H. Galbiati, M. Raasholm, YN. Torres Cleuren, E. Valen, P. Navratilova, EM. Thompson,

. 2019 ; 20 (1) : 908. [pub] 20191129

Language English Country Great Britain

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc20023458

Grant support
183690/S10 NFR-FUGE Norges Forskningsråd
133335/V40 Norges Forskningsråd

BACKGROUND: In phylogenetically diverse organisms, the 5' ends of a subset of mRNAs are trans-spliced with a spliced leader (SL) RNA. The functions of SL trans-splicing, however, remain largely enigmatic. RESULTS: We quantified translation genome-wide in the marine chordate, Oikopleura dioica, under inhibition of mTOR, a central growth regulator. Translation of trans-spliced TOP mRNAs was suppressed, consistent with a role of the SL sequence in nutrient-dependent translational control of growth-related mRNAs. Under crowded, nutrient-limiting conditions, O. dioica continued to filter-feed, but arrested growth until favorable conditions returned. Upon release from unfavorable conditions, initial recovery was independent of nutrient-responsive, trans-spliced genes, suggesting animal density sensing as a first trigger for resumption of development. CONCLUSION: Our results are consistent with a proposed role of trans-splicing in the coordinated translational down-regulation of nutrient-responsive genes under growth-limiting conditions.

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$a Raasholm, Martina $u Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen, Norway. University of Ulm, Ulm, Germany.
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$a Torres Cleuren, Yamila N $u Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen, Norway. Computational Biology Unit, Department of Informatics, University of Bergen, Bergen, Norway.
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$a Valen, Eivind $u Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen, Norway. Computational Biology Unit, Department of Informatics, University of Bergen, Bergen, Norway.
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