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Human Cytomegalovirus Genomes Sequenced Directly From Clinical Material: Variation, Multiple-Strain Infection, Recombination, and Gene Loss
NM. Suárez, GS. Wilkie, E. Hage, S. Camiolo, M. Holton, J. Hughes, M. Maabar, SB. Vattipally, A. Dhingra, UA. Gompels, GWG. Wilkinson, F. Baldanti, M. Furione, D. Lilleri, A. Arossa, T. Ganzenmueller, G. Gerna, P. Hubáček, TF. Schulz, D. Wolf, M....
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
MC_UU_12014/12
Medical Research Council - United Kingdom
MC_UU_12014/3
Medical Research Council - United Kingdom
204870/Z/16/Z
Wellcome Trust - United Kingdom
WT090323MA
Wellcome Trust - United Kingdom
NLK
Free Medical Journals
od 1997 do Před 1 rokem
Open Access Digital Library
od 1997-04-01
PubMed
31050742
DOI
10.1093/infdis/jiz208
Knihovny.cz E-zdroje
- MeSH
- cytomegalovirové infekce virologie MeSH
- Cytomegalovirus genetika MeSH
- databáze nukleových kyselin MeSH
- datové soubory jako téma MeSH
- DNA virů genetika MeSH
- genetická variace MeSH
- genom virový * genetika MeSH
- genotyp MeSH
- lidé MeSH
- molekulární evoluce MeSH
- mutace MeSH
- rekombinace genetická * MeSH
- sekvence nukleotidů * MeSH
- sekvenční analýza DNA MeSH
- sekvenování celého genomu MeSH
- virové geny MeSH
- vysoce účinné nukleotidové sekvenování MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
The genomic characteristics of human cytomegalovirus (HCMV) strains sequenced directly from clinical pathology samples were investigated, focusing on variation, multiple-strain infection, recombination, and gene loss. A total of 207 datasets generated in this and previous studies using target enrichment and high-throughput sequencing were analyzed, in the process enabling the determination of genome sequences for 91 strains. Key findings were that (i) it is important to monitor the quality of sequencing libraries in investigating variation; (ii) many recombinant strains have been transmitted during HCMV evolution, and some have apparently survived for thousands of years without further recombination; (iii) mutants with nonfunctional genes (pseudogenes) have been circulating and recombining for long periods and can cause congenital infection and resulting clinical sequelae; and (iv) intrahost variation in single-strain infections is much less than that in multiple-strain infections. Future population-based studies are likely to continue illuminating the evolution, epidemiology, and pathogenesis of HCMV.
Department of Medical Microbiology Motol University Hospital Prague Czech Republic Israel
Departments of Obstetrics and Gynecology Fondazione IRCCS Policlinico San Matteo Pavia Italy
Division of Infection and Immunity School of Medicine Cardiff University United Kingdom
Institute of Virology Hannover Medical School United Kingdom
Laboratory of Genetics Transplantology and Cardiovascular Diseases Italy
Medical Research Council University of Glasgow Centre for Virus Research United Kingdom
Pathogen Molecular Biology Department London School of Hygiene and Tropical Medicine United Kingdom
Citace poskytuje Crossref.org
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