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Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry
P. Ďurč, V. Dosedělová, F. Foret, J. Dolina, Š. Konečný, M. Himmelsbach, W. Buchberger, P. Kubáň,
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články
Grantová podpora
NV17-31945A
MZ0
CEP - Centrální evidence projektů
- MeSH
- analýza hlavních komponent MeSH
- Barrettův syndrom metabolismus patologie MeSH
- dospělí MeSH
- hmotnostní spektrometrie s elektrosprejovou ionizací metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- pilotní projekty MeSH
- počítačové zpracování signálu MeSH
- referenční standardy MeSH
- reprodukovatelnost výsledků MeSH
- sliny chemie MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- žlučové kyseliny a soli analýza MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
A fast, non-invasive, high-performance liquid chromatographic screening method with electrospray ionization mass spectrometric detection was developed for the analysis of three major glycine-conjugated bile acids in human saliva. Using a mobile phase composed of 80% methanol and 0.1% formic acid, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids were separated in less than 4 minutes with sensitivity in the low nM range. Bile acids are thought to contribute to the pathology of various complications in gastroesophageal reflux disease, for instance, Barrett's esophagus, which may eventually lead to esophageal carcinoma. In this pilot study, samples of saliva obtained from 15 patients with Barrett's esophagus of various severities were compared to saliva samples from 10 healthy volunteers. Glycochenodeoxycholic acid was significantly elevated in the patients and principal component analysis of all bile acids could distinguish the most severe Barrett's esophagus patients. We also reported on the detection of glycochenodeoxycholic acid in exhaled breath condensate for the first time. The promising results of this pilot study warrant future investigation, aiming at non-invasive diagnostics of Barrett's esophagus susceptibility in patients with gastroesophageal reflux disease.
Department of Chemistry Johannes Kepler University Altenbergerstraße 69 4040 Linz Austria
Department of Chemistry Masaryk University Kotlářská 2 611 37 Brno Czech Republic
Citace poskytuje Crossref.org
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- $a Ďurč, Pavol $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00, Brno, Czech Republic; Department of Chemistry, Masaryk University, Kotlářská 2, 611 37, Brno, Czech Republic.
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- $a Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry / $c P. Ďurč, V. Dosedělová, F. Foret, J. Dolina, Š. Konečný, M. Himmelsbach, W. Buchberger, P. Kubáň,
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- $a A fast, non-invasive, high-performance liquid chromatographic screening method with electrospray ionization mass spectrometric detection was developed for the analysis of three major glycine-conjugated bile acids in human saliva. Using a mobile phase composed of 80% methanol and 0.1% formic acid, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids were separated in less than 4 minutes with sensitivity in the low nM range. Bile acids are thought to contribute to the pathology of various complications in gastroesophageal reflux disease, for instance, Barrett's esophagus, which may eventually lead to esophageal carcinoma. In this pilot study, samples of saliva obtained from 15 patients with Barrett's esophagus of various severities were compared to saliva samples from 10 healthy volunteers. Glycochenodeoxycholic acid was significantly elevated in the patients and principal component analysis of all bile acids could distinguish the most severe Barrett's esophagus patients. We also reported on the detection of glycochenodeoxycholic acid in exhaled breath condensate for the first time. The promising results of this pilot study warrant future investigation, aiming at non-invasive diagnostics of Barrett's esophagus susceptibility in patients with gastroesophageal reflux disease.
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- $a Dosedělová, Věra $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00, Brno, Czech Republic.
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- $a Foret, František $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00, Brno, Czech Republic; Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 97, 602 00, Brno, Czech Republic.
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- $a Dolina, Jiří $u Internal Gastroenterology Department, University Hospital Brno, and Faculty of Medicine, Masaryk University, Jihlavská 20, 625 00, Brno, Czech Republic.
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- $a Himmelsbach, Markus $u Department of Chemistry, Johannes-Kepler University, Altenbergerstraße 69, 4040, Linz, Austria.
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- $a Kubáň, Petr $u Department of Bioanalytical Instrumentation, CEITEC Masaryk University, Veveří 97, 602 00, Brno, Czech Republic; Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the Czech Academy of Sciences, Veveří 97, 602 00, Brno, Czech Republic. Electronic address: petr.kuban@ceitec.muni.cz.
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