• Je něco špatně v tomto záznamu ?

Identification of a Diagnostic Set of Endomyocardial Biopsy microRNAs for Acute Cellular Rejection Diagnostics in Patients after Heart Transplantation Using Next-Generation Sequencing

T. Nováková, T. Macháčková, J. Novák, P. Hude, J. Godava, V. Žampachová, J. Oppelt, F. Zlámal, P. Němec, H. Bedáňová, O. Slabý, J. Bienertová-Vašků, L. Špinarová, J. Krejčí,

. 2019 ; 8 (11) : . [pub] 20191106

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20025505

Grantová podpora
NV16-30537A MZ0 CEP - Centrální evidence projektů

INTRODUCTION: Acute cellular rejection (ACR) of heart allografts represents the most common reason for graft failure. Endomyocardial biopsies (EMB) are still subject to substantial interobserver variability. Novel biomarkers enabling precise ACR diagnostics may decrease interobserver variability. We aimed to identify a specific subset of microRNAs reflecting the presence of ACR. PATIENTS AND METHODS: Monocentric retrospective study. A total of 38 patients with the anamnesis of ACR were identified and for each patient three consecutive samples of EMB (with, prior and after ACR) were collected. Sixteen trios were used for next-generation sequencing (exploratory cohort); the resting 22 trios were used for validation with qRT-PCR (validation cohort). Statistical analysis was performed using R software. RESULTS: The analysis of the exploration cohort provided the total of 11 miRNAs that were altered during ACR, the three of which (miR-144, miR-589 and miR-182) were further validated in the validation cohort. Using the levels of all 11 miRNAs and principal component analysis, an ACR score was created with the specificity of 91% and sensitivity of 68% for detecting the presence of ACR in the EMB sample. CONCLUSION: We identified a set of microRNAs altered in endomyocardial biopsies during ACR and using their relative levels we created a diagnostic score that can be used for ACR diagnosis.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc20025505
003      
CZ-PrNML
005      
20250415150523.0
007      
ta
008      
201125s2019 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/cells8111400 $2 doi
035    __
$a (PubMed)31698874
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Nováková, Tereza $u Department of Cardiovascular Diseases, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Pekařská 53, 65691Brno, Czech Republic.
245    10
$a Identification of a Diagnostic Set of Endomyocardial Biopsy microRNAs for Acute Cellular Rejection Diagnostics in Patients after Heart Transplantation Using Next-Generation Sequencing / $c T. Nováková, T. Macháčková, J. Novák, P. Hude, J. Godava, V. Žampachová, J. Oppelt, F. Zlámal, P. Němec, H. Bedáňová, O. Slabý, J. Bienertová-Vašků, L. Špinarová, J. Krejčí,
520    9_
$a INTRODUCTION: Acute cellular rejection (ACR) of heart allografts represents the most common reason for graft failure. Endomyocardial biopsies (EMB) are still subject to substantial interobserver variability. Novel biomarkers enabling precise ACR diagnostics may decrease interobserver variability. We aimed to identify a specific subset of microRNAs reflecting the presence of ACR. PATIENTS AND METHODS: Monocentric retrospective study. A total of 38 patients with the anamnesis of ACR were identified and for each patient three consecutive samples of EMB (with, prior and after ACR) were collected. Sixteen trios were used for next-generation sequencing (exploratory cohort); the resting 22 trios were used for validation with qRT-PCR (validation cohort). Statistical analysis was performed using R software. RESULTS: The analysis of the exploration cohort provided the total of 11 miRNAs that were altered during ACR, the three of which (miR-144, miR-589 and miR-182) were further validated in the validation cohort. Using the levels of all 11 miRNAs and principal component analysis, an ACR score was created with the specificity of 91% and sensitivity of 68% for detecting the presence of ACR in the EMB sample. CONCLUSION: We identified a set of microRNAs altered in endomyocardial biopsies during ACR and using their relative levels we created a diagnostic score that can be used for ACR diagnosis.
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a biologické markery $x metabolismus $7 D015415
650    _2
$a biopsie $x metody $7 D001706
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a rejekce štěpu $x diagnóza $x genetika $x metabolismus $7 D006084
650    _2
$a transplantace srdce $x metody $7 D016027
650    _2
$a vysoce účinné nukleotidové sekvenování $x metody $7 D059014
650    _2
$a lidé $7 D006801
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a mikro RNA $x genetika $7 D035683
650    _2
$a lidé středního věku $7 D008875
650    _2
$a myokard $x metabolismus $7 D009206
650    _2
$a retrospektivní studie $7 D012189
650    _2
$a mladý dospělý $7 D055815
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Macháčková, Táňa $u Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
700    1_
$a Novák, Jan $u Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic. Department of Pathological Physiology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
700    1_
$a Hude, Petr $u Department of Cardiovascular Diseases, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Pekařská 53, 65691Brno, Czech Republic.
700    1_
$a Godava, Július $u Department of Cardiovascular Diseases, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Pekařská 53, 65691Brno, Czech Republic.
700    1_
$a Žampachová, Víta $u Department of Pathology, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Pekařská 53, 65691 Brno, Czech Republic.
700    1_
$a Oppelt, Jan $u Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
700    1_
$a Zlámal, Filip $u Department of Pathological Physiology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
700    1_
$a Němec, Petr $u Centre of Cardiovascular Surgery and Organ Transplantation, Pekařská 53, 65691 Brno, Czech Republic.
700    1_
$a Bedáňová, Helena $u Centre of Cardiovascular Surgery and Organ Transplantation, Pekařská 53, 65691 Brno, Czech Republic.
700    1_
$a Slabý, Ondřej $u Central European Institute of Technology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
700    1_
$a Bienertová-Vašků, Julie $u Department of Pathological Physiology, Masaryk University, Kamenice 5, 62500 Brno, Czech Republic.
700    1_
$a Špinarová, Lenka, $d 1961-2025 $u Department of Cardiovascular Diseases, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Pekařská 53, 65691Brno, Czech Republic. $7 xx0061415
700    1_
$a Krejčí, Jan $u Department of Cardiovascular Diseases, St. Anne's University Hospital and Faculty of Medicine, Masaryk University, Pekařská 53, 65691Brno, Czech Republic.
773    0_
$w MED00194911 $t Cells $x 2073-4409 $g Roč. 8, č. 11 (2019)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31698874 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20201125 $b ABA008
991    __
$a 20250415150529 $b ABA008
999    __
$a ok $b bmc $g 1599650 $s 1116191
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2019 $b 8 $c 11 $e 20191106 $i 2073-4409 $m Cells $n Cells $x MED00194911
GRA    __
$a NV16-30537A $p MZ0
LZP    __
$a Pubmed-20201125

Najít záznam

Citační ukazatele

Možnosti archivace