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Technological advancements for the development of stem cell-based models for hepatotoxicity testing
A. Natale, K. Vanmol, A. Arslan, S. Van Vlierberghe, P. Dubruel, J. Van Erps, H. Thienpont, M. Buzgo, J. Boeckmans, J. De Kock, T. Vanhaecke, V. Rogiers, RM. Rodrigues,
Jazyk angličtina Země Německo
Typ dokumentu časopisecké články, práce podpořená grantem, přehledy
- MeSH
- alternativy testů na zvířatech metody MeSH
- buněčná diferenciace účinky léků MeSH
- buněčné kultury MeSH
- hepatocyty cytologie účinky léků MeSH
- játra účinky léků MeSH
- kmenové buňky cytologie účinky léků MeSH
- lékové postižení jater etiologie MeSH
- lidé MeSH
- xenobiotika toxicita MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
Stem cells are characterized by their self-renewal capacity and their ability to differentiate into multiple cell types of the human body. Using directed differentiation strategies, stem cells can now be converted into hepatocyte-like cells (HLCs) and therefore, represent a unique cell source for toxicological applications in vitro. However, the acquired hepatic functionality of stem cell-derived HLCs is still significantly inferior to primary human hepatocytes. One of the main reasons for this is that most in vitro models use traditional two-dimensional (2D) setups where the flat substrata cannot properly mimic the physiology of the human liver. Therefore, 2D-setups are progressively being replaced by more advanced culture systems, which attempt to replicate the natural liver microenvironment, in which stem cells can better differentiate towards HLCs. This review highlights the most recent cell culture systems, including scaffold-free and scaffold-based three-dimensional (3D) technologies and microfluidics that can be employed for culture and hepatic differentiation of stem cells intended for hepatotoxicity testing. These methodologies have shown to improve in vitro liver cell functionality according to the in vivo liver physiology and allow to establish stem cell-based hepatic in vitro platforms for the accurate evaluation of xenobiotics.
Brussels Photonics Centre of Macromolecular Chemistry Ghent University Ghent Belgium
Brussels Photonics Vrije Universiteit Brussel and Flanders Make Brussels Belgium
Citace poskytuje Crossref.org
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