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Silencing of carbonic anhydrase I enhances the malignant potential of exosomes secreted by prostatic tumour cells

R. Bánová Vulić, M. Zdurienčíková, S. Tyčiaková, O. Benada, M. Dubrovčáková, J. Lakota, Ľ. Škultéty,

. 2019 ; 23 (5) : 3641-3655. [pub] 20190327

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20025877

We report results showing that the silencing of carbonic anhydrase I (siCA1) in prostatic (PC3) tumour cells has a significant impact on exosome formation. An increased diameter, concentration and diversity of the produced exosomes were noticed as a consequence of this knock-down. The protein composition of the exosomes' cargo was also altered. Liquid chromatography and mass spectrometry analyses identified 42 proteins significantly altered in PC3 siCA1 exosomes compared with controls. The affected proteins are mainly involved in metabolic processes, biogenesis, cell component organization and defense/immunity. Interestingly, almost all of them have been described as 'enhancers' of tumour development through the promotion of cell proliferation, migration and invasion. Thus, our results indicate that the reduced expression of the CA1 protein enhances the malignant potential of PC3 cells.

Citace poskytuje Crossref.org

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