Detail
Článek
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results

E. Vrbová, L. Mikalová, L. Grillová, P. Pospíšilová, R. Strnadel, E. Dastychová, M. Kojanová, M. Kreidlová, D. Vaňousová, F. Rob, P. Procházka, A. Krchňáková, V. Vašků, V. Woznicová, M. Dvořáková Heroldová, I. Kuklová, H. Zákoucká, D. Šmajs,

. 2020 ; 15 (8) : e0237949. [pub] 20200820

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc20027927

Grantová podpora
NV17-31333A MZ0 CEP - Centrální evidence projektů

Syphilis, caused by Treponema pallidum ssp. pallidum (TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it is recommended that the final diagnosis be supported by additional tests. The purpose of this study was to analyze the relationship between serology and PCR in syphilis diagnostics. From the year 2004 to May 2019, a total of 941 samples were taken from 833 patients suspected of having syphilis, in Czech Republic. In all these samples, both nested PCR detection of TPA and serology testing were performed. Of the 941 samples, 126 were seronegative, 651 were seropositive, and 164 were serodiscrepant. Among seronegative samples (n = 126), 11 were PCR-positive (8.7%). Among seropositive samples (n = 651; i.e., samples positive for both non-treponemal and treponemal serology tests), 368 samples were PCR-positive (56.5%). The remaining 164 serodiscrepant samples included RPR negative and treponemal serological test-positive samples (n = 154) and a set of 10 RPR-positive samples negative in treponemal serological tests. While the first group revealed 73 PCR-positive samples (47.4%), the second revealed 5 PCR positive samples (50.0%). PCR detection rates were highest in primary syphilis, with lower rates in the secondary and undetermined syphilis stages. As shown here, the nested PCR can improve diagnostics of syphilis, especially in seronegative patients and in patients with discrepant serology.

000      
00000naa a2200000 a 4500
001      
bmc20027927
003      
CZ-PrNML
005      
20211011104205.0
007      
ta
008      
210105s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1371/journal.pone.0237949 $2 doi
035    __
$a (PubMed)32817658
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Vrbová, Eliška $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
245    12
$a A retrospective study on nested PCR detection of syphilis treponemes in clinical samples: PCR detection contributes to the diagnosis of syphilis in patients with seronegative and serodiscrepant results / $c E. Vrbová, L. Mikalová, L. Grillová, P. Pospíšilová, R. Strnadel, E. Dastychová, M. Kojanová, M. Kreidlová, D. Vaňousová, F. Rob, P. Procházka, A. Krchňáková, V. Vašků, V. Woznicová, M. Dvořáková Heroldová, I. Kuklová, H. Zákoucká, D. Šmajs,
520    9_
$a Syphilis, caused by Treponema pallidum ssp. pallidum (TPA), is a persisting global health problem. Although syphilis diagnostics relies mainly on serology, serological tests have some limitations, and it is recommended that the final diagnosis be supported by additional tests. The purpose of this study was to analyze the relationship between serology and PCR in syphilis diagnostics. From the year 2004 to May 2019, a total of 941 samples were taken from 833 patients suspected of having syphilis, in Czech Republic. In all these samples, both nested PCR detection of TPA and serology testing were performed. Of the 941 samples, 126 were seronegative, 651 were seropositive, and 164 were serodiscrepant. Among seronegative samples (n = 126), 11 were PCR-positive (8.7%). Among seropositive samples (n = 651; i.e., samples positive for both non-treponemal and treponemal serology tests), 368 samples were PCR-positive (56.5%). The remaining 164 serodiscrepant samples included RPR negative and treponemal serological test-positive samples (n = 154) and a set of 10 RPR-positive samples negative in treponemal serological tests. While the first group revealed 73 PCR-positive samples (47.4%), the second revealed 5 PCR positive samples (50.0%). PCR detection rates were highest in primary syphilis, with lower rates in the secondary and undetermined syphilis stages. As shown here, the nested PCR can improve diagnostics of syphilis, especially in seronegative patients and in patients with discrepant serology.
650    _2
$a lidé $7 D006801
650    12
$a polymerázová řetězová reakce $7 D016133
650    _2
$a retrospektivní studie $7 D012189
650    _2
$a syfilis $x krev $x diagnóza $7 D013587
650    _2
$a sérologická diagnostika syfilis $x metody $7 D013588
650    _2
$a Treponema $x genetika $x imunologie $x izolace a purifikace $x fyziologie $7 D014208
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Mikalová, Lenka $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Grillová, Linda $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Pospíšilová, Petra $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
700    1_
$a Strnadel, Radim $u Department of Dermatovenerology, Faculty Hospital Brno, Brno, Czech Republic.
700    1_
$a Dastychová, Eliška $u Department of Medical Microbiology, Faculty of Medicine, St. Anne's Hospital and Masaryk University, Brno, Czech Republic.
700    1_
$a Kojanová, Martina $u Department of Dermatology, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
700    1_
$a Kreidlová, Miluše $u Institute of Medical Biochemistry and Laboratory Diagnostics of the General University Hospital and of The First Faculty of Medicine of Charles University in Prague, Prague, Czech Republic. $7 xx0264719
700    1_
$a Vaňousová, Daniela $u Department of Dermatovenerology, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
700    1_
$a Rob, Filip $u Department of Dermatovenerology, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
700    1_
$a Procházka, Přemysl $u Outpatient STI Clinic Medicentrum, Prague, Czech Republic.
700    1_
$a Krchňáková, Alena $u Department of Medical Microbiology, Faculty of Medicine, St. Anne's Hospital and Masaryk University, Brno, Czech Republic.
700    1_
$a Vašků, Vladimír $u Department of Medical Microbiology, Faculty of Medicine, St. Anne's Hospital and Masaryk University, Brno, Czech Republic.
700    1_
$a Woznicová, Vladana $u Department of Medical Microbiology, Faculty of Medicine, St. Anne's Hospital and Masaryk University, Brno, Czech Republic.
700    1_
$a Dvořáková Heroldová, Monika $u Department of Medical Microbiology, Faculty of Medicine, St. Anne's Hospital and Masaryk University, Brno, Czech Republic.
700    1_
$a Kuklová, Ivana $u Department of Dermatology, 1st Faculty of Medicine, Charles University in Prague, Prague, Czech Republic.
700    1_
$a Zákoucká, Hana $u National Reference Laboratory for Diagnostics of the Syphilis, National Institute for Public Health, Prague, Czech Republic.
700    1_
$a Šmajs, David $u Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
773    0_
$w MED00180950 $t PloS one $x 1932-6203 $g Roč. 15, č. 8 (2020), s. e0237949
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32817658 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y a $z 0
990    __
$a 20210105 $b ABA008
991    __
$a 20211011104205 $b ABA008
999    __
$a ok $b bmc $g 1608262 $s 1119107
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 15 $c 8 $d e0237949 $e 20200820 $i 1932-6203 $m PLoS One $n PLoS One $x MED00180950
GRA    __
$a NV17-31333A $p MZ0
LZP    __
$a Pubmed-20210105

Najít záznam

Citační ukazatele

Možnosti archivace