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Oxygen consumption rate of Caenorhabditis elegans as a high-throughput endpoint of toxicity testing using the Seahorse XFe96 Extracellular Flux Analyzer
GD. Preez, H. Fourie, M. Daneel, H. Miller, S. Höss, C. Ricci, G. Engelbrecht, M. Zouhar, V. Wepener,
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články
Free Medical Journals od 2011
Nature Open Access od 2011-12-01
PubMed Central od 2011
Europe PubMed Central od 2011
ProQuest Central od 2011-01-01
Open Access Digital Library od 2011-01-01
Open Access Digital Library od 2011-01-01
Health & Medicine (ProQuest) od 2011-01-01
ROAD: Directory of Open Access Scholarly Resources od 2011
Odkazy
PubMed
32144330
DOI
10.1038/s41598-020-61054-7
Knihovny.cz E-zdroje
- MeSH
- bezpečnost potravin MeSH
- Caenorhabditis elegans účinky léků metabolismus MeSH
- kadmium metabolismus MeSH
- lidé MeSH
- mitochondrie metabolismus MeSH
- nebezpečné látky toxicita MeSH
- průběh práce MeSH
- rychlé screeningové testy metody normy MeSH
- Smegmamorpha * MeSH
- spotřeba kyslíku * MeSH
- testy toxicity metody normy MeSH
- vystavení vlivu životního prostředí MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Caenorhabditis elegans presents functioning, biologically relevant phenotypes and is frequently used as a bioindicator of toxicity. However, most C. elegans in vivo effect-assessment methods are laborious and time consuming. Therefore, we developed a novel method to measure the oxygen consumption rate of C. elegans as a sublethal endpoint of toxicity. This protocol was tested by exposing 50 larval stage one C. elegans individuals for 48 h (at 20 °C) to different concentrations of two toxicants i.e. benzylcetyldimethylammonium chloride (BAC-C16) and cadmium (Cd). Following exposures, the oxygen consumption rate of the C. elegans individuals were measured using the high-throughput functionality of the Seahorse XFe96 Extracellular Flux Analyzer. Dose-response curves for BAC-C16 (R2 = 0.93; P = 0.001) and Cd (R2 = 0.98; P = 0.001) were created. Furthermore, a strong, positive correlation was evidenced between C. elegans oxygen consumption rate and a commonly used, ecologically relevant endpoint of toxicity (growth inhibition) for BAC-C16 (R2 = 0.93; P = 0.0001) and Cd (R2 = 0.91; P = 0.0001). The data presented in this study show that C. elegans oxygen consumption rate can be used as a promising functional measurement of toxicity.
Human Metabolomics Faculty of Natural Sciences North West University Potchefstroom 2520 South Africa
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