-
Something wrong with this record ?
Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry
J. Schejbal, R. Řemínek, Z. Glatz,
Language English Country United States
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Alzheimer Disease drug therapy metabolism MeSH
- Amyloid beta-Protein Precursor metabolism MeSH
- Aspartic Acid Endopeptidases metabolism MeSH
- Electrophoresis, Capillary methods MeSH
- Mass Spectrometry methods MeSH
- Enzyme Inhibitors pharmacology MeSH
- Protease Inhibitors pharmacology MeSH
- Kinetics MeSH
- Humans MeSH
- Mass Screening methods MeSH
- Amyloid Precursor Protein Secretases antagonists & inhibitors MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
Alzheimer's disease is the most common cause of dementia, currently afflicting almost 40 million patients worldwide. According to the amyloid cascade hypothesis, the pathogenesis of the disease could be slowed down or even stopped by the inhibition of beta-secretase, making this aspartic acid protease a potentially important drug target site. Capillary electrophoresis is a promising technique for screening putative enzyme inhibitors due to highly effective separations, minuscule sample and other chemicals consumption, compatibility with a variety of detection techniques, and high throughput via automation. This chapter presents a method based on capillary electrophoresis coupled to mass spectrometry detection for kinetic and inhibition assays of the beta-secretase reaction with a decapeptide derived from an amyloid precursor protein.
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc20028652
- 003
- CZ-PrNML
- 005
- 20210114154600.0
- 007
- ta
- 008
- 210105s2020 xxu f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1007/978-1-0716-0163-1_9 $2 doi
- 035 __
- $a (PubMed)31773652
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxu
- 100 1_
- $a Schejbal, Jan $u Faculty of Science, Department of Biochemistry, Masaryk University, Brno, Czech Republic. honzaschejbal@mail.muni.cz.
- 245 10
- $a Screening of Beta-Secretase Inhibitors by Capillary Electrophoresis-Mass Spectrometry / $c J. Schejbal, R. Řemínek, Z. Glatz,
- 520 9_
- $a Alzheimer's disease is the most common cause of dementia, currently afflicting almost 40 million patients worldwide. According to the amyloid cascade hypothesis, the pathogenesis of the disease could be slowed down or even stopped by the inhibition of beta-secretase, making this aspartic acid protease a potentially important drug target site. Capillary electrophoresis is a promising technique for screening putative enzyme inhibitors due to highly effective separations, minuscule sample and other chemicals consumption, compatibility with a variety of detection techniques, and high throughput via automation. This chapter presents a method based on capillary electrophoresis coupled to mass spectrometry detection for kinetic and inhibition assays of the beta-secretase reaction with a decapeptide derived from an amyloid precursor protein.
- 650 _2
- $a Alzheimerova nemoc $x farmakoterapie $x metabolismus $7 D000544
- 650 _2
- $a sekretasy $x antagonisté a inhibitory $7 D053829
- 650 _2
- $a amyloidový prekurzorový protein beta $x metabolismus $7 D016564
- 650 _2
- $a aspartátové endopeptidasy $x metabolismus $7 D016282
- 650 _2
- $a elektroforéza kapilární $x metody $7 D019075
- 650 _2
- $a inhibitory enzymů $x farmakologie $7 D004791
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a kinetika $7 D007700
- 650 _2
- $a plošný screening $x metody $7 D008403
- 650 _2
- $a hmotnostní spektrometrie $x metody $7 D013058
- 650 _2
- $a inhibitory proteas $x farmakologie $7 D011480
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Řemínek, Roman $u Faculty of Science, Department of Biochemistry, Masaryk University, Brno, Czech Republic.
- 700 1_
- $a Glatz, Zdeněk $u Faculty of Science, Department of Biochemistry, Masaryk University, Brno, Czech Republic.
- 773 0_
- $w MED00180389 $t Methods in molecular biology (Clifton, N.J.) $x 1940-6029 $g Roč. 2089, č. - (2020), s. 147-157
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/31773652 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y a $z 0
- 990 __
- $a 20210105 $b ABA008
- 991 __
- $a 20210114154557 $b ABA008
- 999 __
- $a ok $b bmc $g 1608987 $s 1119832
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2020 $b 2089 $c - $d 147-157 $e - $i 1940-6029 $m Methods in molecular biology $n Methods Mol Biol $x MED00180389
- LZP __
- $a Pubmed-20210105
