Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs

H. Zhu, VH. Blahnová, G. Perale, J. Xiao, F. Betge, F. Boniolo, E. Filová, SP. Lyngstadaas, HJ. Haugen

. 2020 ; 8 (-) : 619111. [pub] 20201222

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc21010206

Bone defect is a noteworthy health problem and is the second most transplanted tissue after blood. Numerous bone grafts are designed and applied in clinics. Limitations, however, from different aspects still exist, including limited supply, mechanical strength, and bioactivity. In this study, two biomimetic peptides (P2 and P6) are incorporated into a composite bioactive xeno hybrid bone graft named SmartBonePep®, with the aim to increase the bioactivity of the bone graft. The results, which include cytotoxicity, proliferation rate, confocal microscopy, gene expression, and protein qualification, successfully prove that the SmartBonePep® has multi-modal biological effects on human mesenchymal stem cells from bone marrow. The effective physical entrapment of P6 into a composite xeno-hybrid bone graft, withstanding manufacturing processes including exposure to strong organic solvents and ethylene oxide sterilization, increases the osteogenic potential of the stem cells as well as cell attachment and proliferation. P2 and P6 both show a strong biological potential and may be future candidates for enhancing the clinical performance of bone grafts.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21010206
003      
CZ-PrNML
005      
20210715081246.0
007      
ta
008      
210413s2020 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3389/fcell.2020.619111 $2 doi
035    __
$a (PubMed)33415112
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Zhu, Hao $u Department of Orthopedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
245    10
$a Xeno-Hybrid Bone Graft Releasing Biomimetic Proteins Promotes Osteogenic Differentiation of hMSCs / $c H. Zhu, VH. Blahnová, G. Perale, J. Xiao, F. Betge, F. Boniolo, E. Filová, SP. Lyngstadaas, HJ. Haugen
520    9_
$a Bone defect is a noteworthy health problem and is the second most transplanted tissue after blood. Numerous bone grafts are designed and applied in clinics. Limitations, however, from different aspects still exist, including limited supply, mechanical strength, and bioactivity. In this study, two biomimetic peptides (P2 and P6) are incorporated into a composite bioactive xeno hybrid bone graft named SmartBonePep®, with the aim to increase the bioactivity of the bone graft. The results, which include cytotoxicity, proliferation rate, confocal microscopy, gene expression, and protein qualification, successfully prove that the SmartBonePep® has multi-modal biological effects on human mesenchymal stem cells from bone marrow. The effective physical entrapment of P6 into a composite xeno-hybrid bone graft, withstanding manufacturing processes including exposure to strong organic solvents and ethylene oxide sterilization, increases the osteogenic potential of the stem cells as well as cell attachment and proliferation. P2 and P6 both show a strong biological potential and may be future candidates for enhancing the clinical performance of bone grafts.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Blahnová, Veronika Hefka $u Department of Tissue Engineering, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czechia ; Department of Biophysics, Second Faculty of Medicine, Charles University, Prague, Czechia
700    1_
$a Perale, Giuseppe $u Industrie Biomediche Insubri S.A., Mezzovico-Vira, Switzerland ; Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Vienna, Austria ; Faculty of Biomedical Sciences, University of Southern Switzerland, Lugano, Switzerland
700    1_
$a Xiao, Jun $u Department of Orthopedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
700    1_
$a Betge, Felice $u Industrie Biomediche Insubri S.A., Mezzovico-Vira, Switzerland
700    1_
$a Boniolo, Fabio $u Helmholtz Zentrum München Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH), Neuherberg, Germany
700    1_
$a Filová, Eva $u Department of Tissue Engineering, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czechia ; Department of Biophysics, Second Faculty of Medicine, Charles University, Prague, Czechia
700    1_
$a Lyngstadaas, Ståle Petter $u Corticalis AS, Oslo, Norway ; Department of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, Norway
700    1_
$a Haugen, Håvard Jostein $u Corticalis AS, Oslo, Norway ; Department of Biomaterials, Faculty of Dentistry, University of Oslo, Oslo, Norway
773    0_
$w MED00186212 $t Frontiers in cell and developmental biology $x 2296-634X $g Roč. 8, č. - (2020), s. 619111
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33415112 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20210413 $b ABA008
991    __
$a 20210715081245 $b ABA008
999    __
$a ind $b bmc $g 1649722 $s 1130582
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 8 $c - $d 619111 $e 20201222 $i 2296-634X $m Frontiers in cell and developmental biology. $n Front Cell Dev Biol $x MED00186212
LZP    __
$a Pubmed-20210413

Find record

Citation metrics

Archiving options