Dielectric particles in a non-uniform electric field are subject to a force caused by a phenomenon called dielectrophoresis (DEP). DEP is a commonly used technique in microfluidics for particle or cell separation. In comparison with other separation methods, DEP has the unique advantage of being label-free, fast, and accurate. It has been widely applied in microfluidics for bio-molecular diagnostics and medical and polymer research. This review introduces the basic theory of DEP, its advantages compared with other separation methods, and its applications in recent years, in particular, focusing on the different electrode types integrated into microfluidic chips, fabrication techniques, and operation principles.

Central European Institute of Technology Brno University of Technology Brno 61300 Czech Republic

Department of Microelectronics Faculty of Electrical Engineering Brno University of Technology Technická 3058 10 Brno 61600 Czech Republic

Ministry of Education Key Laboratory of Micro Nano Systems for Aerospace School of Mechanical Engineering Northwestern Polytechnical University 127 West Youyi Road Xi'an 710072 China

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