-
Je něco špatně v tomto záznamu ?
Longitudinal high-throughput TCR repertoire profiling reveals the dynamics of T-cell memory formation after mild COVID-19 infection
AA. Minervina, EA. Komech, A. Titov, M. Bensouda Koraichi, E. Rosati, IZ. Mamedov, A. Franke, GA. Efimov, DM. Chudakov, T. Mora, AM. Walczak, YB. Lebedev, MV. Pogorelyy
Jazyk angličtina Země Velká Británie
Typ dokumentu časopisecké články, práce podpořená grantem
Grantová podpora
RSF 20-15-00351
Russian Science Foundation - International
Exc2167
Deutsche Forschungsgemeinschaft - International
4096610003
Deutsche Forschungsgemeinschaft - International
COG 724208
H2020 European Research Council - International
19-54-12-011
Russian Foundation for Basic Research - International
18-19-09132
Russian Foundation for Basic Research - International
075-15-2019-1789
Ministry of Science and Higher Education of the Russian Federation - International
075-15-2019-1789
Ministry of Science and Higher Education - International
Free Medical Journals od 2012
PubMed Central od 2012
Europe PubMed Central od 2012
ProQuest Central od 2012-01-01
Open Access Digital Library od 2012-01-01
Open Access Digital Library od 2013-01-01
Health & Medicine (ProQuest) od 2012-01-01
ROAD: Directory of Open Access Scholarly Resources od 2012
Odkazy
PubMed
33399535
DOI
10.7554/elife.63502
Knihovny.cz E-zdroje
- MeSH
- COVID-19 imunologie patofyziologie MeSH
- genová knihovna MeSH
- imunologická paměť * MeSH
- lidé MeSH
- longitudinální studie MeSH
- mapování epitopu MeSH
- receptory antigenů T-buněk chemie genetika MeSH
- SARS-CoV-2 fyziologie MeSH
- sekvence aminokyselin MeSH
- stupeň závažnosti nemoci MeSH
- T-lymfocyty imunologie MeSH
- testování histokompatibility MeSH
- zkřížené reakce MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
COVID-19 is a global pandemic caused by the SARS-CoV-2 coronavirus. T cells play a key role in the adaptive antiviral immune response by killing infected cells and facilitating the selection of virus-specific antibodies. However, neither the dynamics and cross-reactivity of the SARS-CoV-2-specific T-cell response nor the diversity of resulting immune memory is well understood. In this study, we use longitudinal high-throughput T-cell receptor (TCR) sequencing to track changes in the T-cell repertoire following two mild cases of COVID-19. In both donors, we identified CD4+ and CD8+ T-cell clones with transient clonal expansion after infection. We describe characteristic motifs in TCR sequences of COVID-19-reactive clones and show preferential occurrence of these motifs in publicly available large dataset of repertoires from COVID-19 patients. We show that in both donors, the majority of infection-reactive clonotypes acquire memory phenotypes. Certain T-cell clones were detected in the memory fraction at the pre-infection time point, suggesting participation of pre-existing cross-reactive memory T cells in the immune response to SARS-CoV-2.
Institute of Clinical Molecular Biology Kiel University Kiel Germany
Masaryk University Central European Institute of Technology Brno Czech Republic
Moscow State University Moscow Russian Federation
National Research Center for Hematology Moscow Russian Federation
Pirogov Russian National Research Medical University Moscow Russian Federation
Shemyakin Ovchinnikov Institute of Bioorganic Chemistry Moscow Russian Federation
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21011626
- 003
- CZ-PrNML
- 005
- 20210714092552.0
- 007
- ta
- 008
- 210420s2021 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.7554/eLife.63502 $2 doi
- 035 __
- $a (PubMed)33399535
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Minervina, Anastasia A $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation
- 245 10
- $a Longitudinal high-throughput TCR repertoire profiling reveals the dynamics of T-cell memory formation after mild COVID-19 infection / $c AA. Minervina, EA. Komech, A. Titov, M. Bensouda Koraichi, E. Rosati, IZ. Mamedov, A. Franke, GA. Efimov, DM. Chudakov, T. Mora, AM. Walczak, YB. Lebedev, MV. Pogorelyy
- 520 9_
- $a COVID-19 is a global pandemic caused by the SARS-CoV-2 coronavirus. T cells play a key role in the adaptive antiviral immune response by killing infected cells and facilitating the selection of virus-specific antibodies. However, neither the dynamics and cross-reactivity of the SARS-CoV-2-specific T-cell response nor the diversity of resulting immune memory is well understood. In this study, we use longitudinal high-throughput T-cell receptor (TCR) sequencing to track changes in the T-cell repertoire following two mild cases of COVID-19. In both donors, we identified CD4+ and CD8+ T-cell clones with transient clonal expansion after infection. We describe characteristic motifs in TCR sequences of COVID-19-reactive clones and show preferential occurrence of these motifs in publicly available large dataset of repertoires from COVID-19 patients. We show that in both donors, the majority of infection-reactive clonotypes acquire memory phenotypes. Certain T-cell clones were detected in the memory fraction at the pre-infection time point, suggesting participation of pre-existing cross-reactive memory T cells in the immune response to SARS-CoV-2.
- 650 _2
- $a sekvence aminokyselin $7 D000595
- 650 _2
- $a COVID-19 $x imunologie $x patofyziologie $7 D000086382
- 650 _2
- $a zkřížené reakce $7 D003429
- 650 _2
- $a mapování epitopu $7 D018604
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a genová knihovna $7 D015723
- 650 _2
- $a testování histokompatibility $7 D006650
- 650 _2
- $a lidé $7 D006801
- 650 12
- $a imunologická paměť $7 D007156
- 650 _2
- $a longitudinální studie $7 D008137
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a receptory antigenů T-buněk $x chemie $x genetika $7 D011948
- 650 _2
- $a SARS-CoV-2 $x fyziologie $7 D000086402
- 650 _2
- $a stupeň závažnosti nemoci $7 D012720
- 650 _2
- $a T-lymfocyty $x imunologie $7 D013601
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Komech, Ekaterina A $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation ; Pirogov Russian National Research Medical University, Moscow, Russian Federation
- 700 1_
- $a Titov, Aleksei $u National Research Center for Hematology, Moscow, Russian Federation
- 700 1_
- $a Bensouda Koraichi, Meriem $u Laboratoire de physique de l'École Normale Supérieure, ENS, PSL, Sorbonne Universite, Universite de Paris, and CNRS, Paris, France
- 700 1_
- $a Rosati, Elisa $u Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany
- 700 1_
- $a Mamedov, Ilgar Z $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation $u Pirogov Russian National Research Medical University, Moscow, Russian Federation $u Masaryk University, Central European Institute of Technology, Brno, Czech Republic $u V.I. Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Moscow, Russian Federation
- 700 1_
- $a Franke, Andre $u Institute of Clinical Molecular Biology, Kiel University, Kiel, Germany
- 700 1_
- $a Efimov, Grigory A $u National Research Center for Hematology, Moscow, Russian Federation
- 700 1_
- $a Chudakov, Dmitriy M $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation $u Pirogov Russian National Research Medical University, Moscow, Russian Federation $u Masaryk University, Central European Institute of Technology, Brno, Czech Republic
- 700 1_
- $a Mora, Thierry $u Laboratoire de physique de l'École Normale Supérieure, ENS, PSL, Sorbonne Universite, Universite de Paris, and CNRS, Paris, France
- 700 1_
- $a Walczak, Aleksandra M $u Laboratoire de physique de l'École Normale Supérieure, ENS, PSL, Sorbonne Universite, Universite de Paris, and CNRS, Paris, France
- 700 1_
- $a Lebedev, Yuri B $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation ; Moscow State University, Moscow, Russian Federation
- 700 1_
- $a Pogorelyy, Mikhail V $u Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russian Federation ; Pirogov Russian National Research Medical University, Moscow, Russian Federation
- 773 0_
- $w MED00188753 $t eLife $x 2050-084X $g Roč. 10, č. - (2021)
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33399535 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20210420 $b ABA008
- 991 __
- $a 20210714092550 $b ABA008
- 999 __
- $a ok $b bmc $g 1650099 $s 1132005
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 10 $c - $e 20210105 $i 2050-084X $m eLife $n eLife $x MED00188753
- GRA __
- $a RSF 20-15-00351 $p Russian Science Foundation $2 International
- GRA __
- $a Exc2167 $p Deutsche Forschungsgemeinschaft $2 International
- GRA __
- $a 4096610003 $p Deutsche Forschungsgemeinschaft $2 International
- GRA __
- $a COG 724208 $p H2020 European Research Council $2 International
- GRA __
- $a 19-54-12-011 $p Russian Foundation for Basic Research $2 International
- GRA __
- $a 18-19-09132 $p Russian Foundation for Basic Research $2 International
- GRA __
- $a 075-15-2019-1789 $p Ministry of Science and Higher Education of the Russian Federation $2 International
- GRA __
- $a 075-15-2019-1789 $p Ministry of Science and Higher Education $2 International
- LZP __
- $a Pubmed-20210420