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Aesculus hippocastanum L. Extract Does Not Induce Fibroblast to Myofibroblast Conversion but Increases Extracellular Matrix Production In Vitro Leading to Increased Wound Tensile Strength in Rats
I. Kováč, N. Melegová, M. Čoma, P. Takáč, K. Kováčová, M. Hollý, J. Ďurkáč, L. Urban, M. Gurbáľová, E. Švajdlenka, G. Mojžišová, R. Zajíček, P. Szabo, P. Mučaji, P. Gál
Language English Country Switzerland
Document type Journal Article
Grant support
VEGA No. 1/0561/18 and VEGA 1/0319/20
Vedecká Grantová Agentúra MŠVVaŠ SR a SAV
APVV-16-0446
Agentúra na Podporu Výskumu a Vývoja
No. CZ.1.05/1.1.00/02.0109
European Regional Development Fund
ITMS2014 and 313011D103
European Regional Development Fund
PROGRES Q37 and Q28
Univerzita Karlova v Praze
NLK
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- MeSH
- Aesculus chemistry MeSH
- Extracellular Matrix drug effects metabolism MeSH
- Wound Healing drug effects MeSH
- Rats MeSH
- Molecular Structure MeSH
- Myofibroblasts drug effects metabolism MeSH
- Tensile Strength MeSH
- Regeneration MeSH
- Plant Extracts chemistry pharmacology MeSH
- Chromatography, High Pressure Liquid MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
The ability of horse chestnut extract (HCE) to induce contraction force in fibroblasts, a process with remarkable significance in skin repair, motivated us to evaluate its wound healing potential in a series of experiments. In the in vitro study of the ability of human dermal fibroblasts to form myofibroblast-like cells was evaluated at the protein level (Western blot and immunofluorescence). The in vivo study was conducted on male Sprague-Dawley rats with inflicted wounds (one open circular and one sutured incision) on their backs. Rats were topically treated with two tested HCE concentrations (0.1% and 1%) or sterile water. The control group remained untreated. The incisions were processed for wound tensile strength (TS) measurement whereas the open wounds were subjected to histological examination. On the in vitro level the HCE extract induced fibronectin-rich extracellular matrix formation, but did not induced α-smooth muscle actin (SMA) expression in dermal fibroblasts. The animal study revealed that HCE increased wound TS and improved collagen organization. In conclusion, the direct comparison of both basic wound models demonstrated that the healing was significantly increased following HCE, thus this extract may be found useful to improve healing of acute wounds. Nevertheless, the use of an experimental rat model warrants a direct extrapolation to the human clinical situation.
Department of Pathology Louise Pasteur University Hospital 041 90 Košice Slovakia
Department of Pharmacology Faculty of Medicine Pavol Jozef Šafárik University 040 11 Košice Slovakia
Institute of Anatomy 1st Faculty of Medicine Charles University 128 00 Prague Czech Republic
References provided by Crossref.org
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- $a The ability of horse chestnut extract (HCE) to induce contraction force in fibroblasts, a process with remarkable significance in skin repair, motivated us to evaluate its wound healing potential in a series of experiments. In the in vitro study of the ability of human dermal fibroblasts to form myofibroblast-like cells was evaluated at the protein level (Western blot and immunofluorescence). The in vivo study was conducted on male Sprague-Dawley rats with inflicted wounds (one open circular and one sutured incision) on their backs. Rats were topically treated with two tested HCE concentrations (0.1% and 1%) or sterile water. The control group remained untreated. The incisions were processed for wound tensile strength (TS) measurement whereas the open wounds were subjected to histological examination. On the in vitro level the HCE extract induced fibronectin-rich extracellular matrix formation, but did not induced α-smooth muscle actin (SMA) expression in dermal fibroblasts. The animal study revealed that HCE increased wound TS and improved collagen organization. In conclusion, the direct comparison of both basic wound models demonstrated that the healing was significantly increased following HCE, thus this extract may be found useful to improve healing of acute wounds. Nevertheless, the use of an experimental rat model warrants a direct extrapolation to the human clinical situation.
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