Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Glucose, Cyc8p and Tup1p regulate biofilm formation and dispersal in wild Saccharomyces cerevisiae

P. Van Nguyen, V. Plocek, L. Váchová, Z. Palková

. 2020 ; 6 (1) : 7. [pub] 20200213

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21012863

Saccharomyces cerevisiae is a mainly beneficial yeast, widely used in the food industry. However, there is growing evidence of its potential pathogenicity, leading to fungemia and invasive infections. The medical impact of yeast pathogens depends on formation of biofilms: multicellular structures, protected from the environment. Cell adhesion is a prerequisite of biofilm formation. We investigated the adherence of wild and genetically modified S. cerevisiae strains, formation of solid-liquid interface biofilms and associated regulation. Planktonic and static cells of wild strain BRF adhered and formed biofilms in glucose-free medium. Tup1p and Cyc8p were key positive and negative regulators, respectively. Glucose caused increased Cyc8p levels and blocked cell adhesion. Even low glucose levels, comparable with levels in the blood, allowed biofilm dispersal and release of planktonic cells. Cyc8p could thus modulate cell adhesion in different niches, dependently on environmental glucose level, e.g., high-glucose blood versus low-glucose tissues in host organisms.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21012863
003      
CZ-PrNML
005      
20210507104000.0
007      
ta
008      
210420s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41522-020-0118-1 $2 doi
035    __
$a (PubMed)32054862
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Van Nguyen, Phu $u Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic
245    10
$a Glucose, Cyc8p and Tup1p regulate biofilm formation and dispersal in wild Saccharomyces cerevisiae / $c P. Van Nguyen, V. Plocek, L. Váchová, Z. Palková
520    9_
$a Saccharomyces cerevisiae is a mainly beneficial yeast, widely used in the food industry. However, there is growing evidence of its potential pathogenicity, leading to fungemia and invasive infections. The medical impact of yeast pathogens depends on formation of biofilms: multicellular structures, protected from the environment. Cell adhesion is a prerequisite of biofilm formation. We investigated the adherence of wild and genetically modified S. cerevisiae strains, formation of solid-liquid interface biofilms and associated regulation. Planktonic and static cells of wild strain BRF adhered and formed biofilms in glucose-free medium. Tup1p and Cyc8p were key positive and negative regulators, respectively. Glucose caused increased Cyc8p levels and blocked cell adhesion. Even low glucose levels, comparable with levels in the blood, allowed biofilm dispersal and release of planktonic cells. Cyc8p could thus modulate cell adhesion in different niches, dependently on environmental glucose level, e.g., high-glucose blood versus low-glucose tissues in host organisms.
650    _2
$a bakteriální adheze $7 D001422
650    _2
$a biofilmy $x růst a vývoj $7 D018441
650    _2
$a kultivační média $x chemie $7 D003470
650    _2
$a regulace genové exprese u hub $7 D015966
650    _2
$a glukosa $x metabolismus $7 D005947
650    _2
$a mutace $7 D009154
650    _2
$a jaderné proteiny $x genetika $x metabolismus $7 D009687
650    _2
$a represorové proteiny $x genetika $x metabolismus $7 D012097
650    _2
$a Saccharomyces cerevisiae $x genetika $x fyziologie $7 D012441
650    _2
$a Saccharomyces cerevisiae - proteiny $x genetika $x metabolismus $7 D029701
650    _2
$a povrchové vlastnosti $7 D013499
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Plocek, Vítězslav $u Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic
700    1_
$a Váchová, Libuše $u Institute of Microbiology of the Czech Academy of Sciences, BIOCEV, Prague, Czech Republic
700    1_
$a Palková, Zdena $u Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic. zdenap@natur.cuni.cz
773    0_
$w MED00197303 $t NPJ biofilms and microbiomes $x 2055-5008 $g Roč. 6, č. 1 (2020), s. 7
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32054862 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210420 $b ABA008
991    __
$a 20210507103959 $b ABA008
999    __
$a ok $b bmc $g 1651106 $s 1133242
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 6 $c 1 $d 7 $e 20200213 $i 2055-5008 $m NPJ biofilms and microbiomes $n NPJ Biofilms Microbiomes $x MED00197303
LZP    __
$a Pubmed-20210420

Najít záznam

Citační ukazatele

Možnosti archivace