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Micropropagation of Rosaceous Species SAM Grown in Temperate Climate
J. Sedlak, F. Paprstein
Language English Country United States
Document type Journal Article
- MeSH
- Acclimatization physiology MeSH
- Cytokinins pharmacology MeSH
- Culture Media chemistry pharmacology MeSH
- Indoleacetic Acids pharmacology MeSH
- Meristem drug effects growth & development MeSH
- Cell Proliferation drug effects physiology MeSH
- Plant Growth Regulators pharmacology MeSH
- Rosaceae drug effects growth & development MeSH
- In Vitro Techniques MeSH
- Tissue Culture Techniques methods MeSH
- Plant Shoots drug effects growth & development MeSH
- Publication type
- Journal Article MeSH
The benefits of in vitro plant cultivation are mainly due to very high multiplication rate. Cultivation of plant material in vitro can be carried out during the whole year regardless of the time of the year or weather conditions. We create artificial conditions in the lab (heat, light, humidity), and we can regulate these conditions at any time. For the preservation of cultivar identity, we recommend establishing in vitro cultures from shoot tips usually larger than 0.2 mm. In practice, in vitro cultivation of plants uses these growth regulators to achieve organogenesis, for example, root formation, prolonged growth, or multiplication. During each subculture, these cultures are then transferred on a solid agar medium in the form of actively growing multiple shoots with a well-differentiated shoot tip containing meristematic area. Cytokinins are important for cell division and causes branching of plants. Auxins, both endogenous and exogenous, act at as a trigger for the differentiation and formation of root primordia. Morphological characteristics (formation of leaves or callus) and shoot development should be observed during in vitro multiplication and after transfer to ex vitro conditions.
References provided by Crossref.org
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