• Something wrong with this record ?

Methodology of deconvolution of total solute retention on chemically modified stationary phases to structure specific contributions of bound compounds

B. Dolenský, A. Tatar, M. Havlík, T. Navrátilová, J. Hajduch, P. Matějka

. 2021 ; 1642 (-) : 462030. [pub] 20210303

Language English Country Netherlands

Document type Journal Article

The total solute retention by a chemically modified stationary phase (CMSP) has been shown several times to be a potential tool for studying the binding abilities of the bound compound. In this article, we present a methodology for the deconvolution of the total retention into structure-specific contributions. Three complementary silica-based CMSPs were prepared: 1) non-modified silica, 2) silica modified by syn-bis-Tröger's base (a molecular tweezer) and 3) silica modified by anti-bis-Tröger's base (a non-tweezer molecule). These were characterized by elemental analysis and Raman spectroscopy, and used to assemble liquid chromatography (LC) columns. The total retention factors were estimated for electron-deficient nitro- and cyano-derivatives of benzene in both normal and reverse elution modes. The total retention factor was considered to be the sum of structure-specific retention factors, each related to the affinity (the binding constant) of a specific structure (the binding site), and its content in the modified silica, as defined for weak-affinity chromatography (WAC). The obtained structure-specific contributions are in line with the binding studies of ligands in solution. They reveal details of the retention mechanism, suggesting a more suitable attachment of ligands, and expose the shortcomings of evaluations based solely on the total retentions.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21018844
003      
CZ-PrNML
005      
20210830100428.0
007      
ta
008      
210728s2021 ne f 000 0|eng||
009      
AR
024    7_
$a 10.1016/j.chroma.2021.462030 $2 doi
035    __
$a (PubMed)33721812
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a ne
100    1_
$a Dolenský, Bohumil $u Department of Analytical Chemistry, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha, Czech Republic. Electronic address: Bohumil.Dolensky@vscht.cz
245    10
$a Methodology of deconvolution of total solute retention on chemically modified stationary phases to structure specific contributions of bound compounds / $c B. Dolenský, A. Tatar, M. Havlík, T. Navrátilová, J. Hajduch, P. Matějka
520    9_
$a The total solute retention by a chemically modified stationary phase (CMSP) has been shown several times to be a potential tool for studying the binding abilities of the bound compound. In this article, we present a methodology for the deconvolution of the total retention into structure-specific contributions. Three complementary silica-based CMSPs were prepared: 1) non-modified silica, 2) silica modified by syn-bis-Tröger's base (a molecular tweezer) and 3) silica modified by anti-bis-Tröger's base (a non-tweezer molecule). These were characterized by elemental analysis and Raman spectroscopy, and used to assemble liquid chromatography (LC) columns. The total retention factors were estimated for electron-deficient nitro- and cyano-derivatives of benzene in both normal and reverse elution modes. The total retention factor was considered to be the sum of structure-specific retention factors, each related to the affinity (the binding constant) of a specific structure (the binding site), and its content in the modified silica, as defined for weak-affinity chromatography (WAC). The obtained structure-specific contributions are in line with the binding studies of ligands in solution. They reveal details of the retention mechanism, suggesting a more suitable attachment of ligands, and expose the shortcomings of evaluations based solely on the total retentions.
650    _2
$a chromatografie kapalinová $x metody $7 D002853
650    _2
$a ligandy $7 D008024
650    _2
$a oxid křemičitý $x chemie $7 D012822
650    _2
$a roztoky $7 D012996
650    _2
$a stereoizomerie $7 D013237
655    _2
$a časopisecké články $7 D016428
700    1_
$a Tatar, Ameneh $u Department of Analytical Chemistry, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha, Czech Republic
700    1_
$a Havlík, Martin $u Department of Analytical Chemistry, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha, Czech Republic
700    1_
$a Navrátilová, Tereza $u Department of Analytical Chemistry, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha, Czech Republic
700    1_
$a Hajduch, Jan $u Department of Analytical Chemistry, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha, Czech Republic
700    1_
$a Matějka, Pavel $u Department of Analytical Chemistry, University of Chemistry and Technology in Prague, Technická 5, 166 28 Praha, Czech Republic
773    0_
$w MED00004962 $t Journal of chromatography. A $x 1873-3778 $g Roč. 1642, č. - (2021), s. 462030
856    41
$u https://pubmed.ncbi.nlm.nih.gov/33721812 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210728 $b ABA008
991    __
$a 20210830100428 $b ABA008
999    __
$a ok $b bmc $g 1689819 $s 1139290
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 1642 $c - $d 462030 $e 20210303 $i 1873-3778 $m Journal of chromatography. A, Including electrophoresis and other separation methods $n J Chromatogr A $x MED00004962
LZP    __
$a Pubmed-20210728

Find record

Citation metrics

Loading data ...

Archiving options

Loading data ...