-
Something wrong with this record ?
The dysregulation of monocyte subpopulations in individuals at risk of developing rheumatoid arthritis
K. Prajzlerová, O. Kryštůfková, M. Komarc, H. Mann, H. Hulejová, N. Petrovská, M. Gregová, P. Hánová, K. Pavelka, J. Vencovský, L. Šenolt, M. Filková
Language English Country Great Britain
Document type Journal Article, Research Support, Non-U.S. Gov't
Grant support
NV17-32612A
MZ0
CEP Register
Digital library NLK
Full text - Article
NLK
Free Medical Journals
from 1996 to 1 year ago
Open Access Digital Library
from 1996-01-01
Medline Complete (EBSCOhost)
from 1999-01-01 to 1 year ago
- MeSH
- Arthralgia etiology MeSH
- C-Reactive Protein analysis MeSH
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Monocytes metabolism MeSH
- Follow-Up Studies MeSH
- Disease Progression MeSH
- Anti-Citrullinated Protein Antibodies blood MeSH
- Arthritis, Rheumatoid blood diagnosis MeSH
- Case-Control Studies MeSH
- Check Tag
- Adult MeSH
- Middle Aged MeSH
- Humans MeSH
- Male MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
OBJECTIVES: Individuals carrying antibodies against citrullinated proteins (ACPA) are at high risk of developing RA. EULAR provided a clinical definition of individuals with arthralgia suspicious for progression to RA (clinically suspect arthralgia, CSA). The alteration of monocyte subpopulations in patients with established RA has been previously described. We analysed peripheral blood monocyte subpopulations in individuals with arthralgia at risk of RA. METHODS: We included 70 at-risk individuals, defined as having arthralgia without arthritis and being either ACPA+ or meeting the clinical CSA definition, 23 patients with early RA (ERA) and 19 healthy controls (HCs). Monocytes classified as classical (CD14++CD16-), intermediate (CD14++CD16+/++) and nonclassical (CD14-/+CD16++) were analysed by flow cytometry. RESULTS: Of the 70 at-risk individuals, 46 were ACPA+ and 45 met the CSA definition. The at-risk individuals and, especially, ERA patients had a lower percentage of classical monocytes and a higher percentage of nonclassical monocytes than the HCs. ACPA positivity had no effect on the difference in the distribution of the monocyte subsets between at-risk individuals and ERA patients, but a difference was determined in those reaching the ERA phase. However, when compared with HCs, the shift of monocyte subsets was more significant in ACPA+ than in ACPA- individuals with arthralgia. This trend was observed in individuals who did not meet the CSA definition. This finding was, however, determined by a selection bias, as these individuals were solely ACPA+. CONCLUSION: The shift from classical to nonclassical monocyte subpopulations was observed already in individuals at risk of developing RA.
Department of Experimental Rheumatology Institute of Rheumatology Prague Czech Republic
Department of Rheumatology 1st Faculty of Medicine Charles University Prague Czech Republic
References provided by Crossref.org
- 000
- 00000naa a2200000 a 4500
- 001
- bmc21018865
- 003
- CZ-PrNML
- 005
- 20210914145618.0
- 007
- ta
- 008
- 210728s2021 xxk f 000 0|eng||
- 009
- AR
- 024 7_
- $a 10.1093/rheumatology/keaa518 $2 doi
- 035 __
- $a (PubMed)33119082
- 040 __
- $a ABA008 $b cze $d ABA008 $e AACR2
- 041 0_
- $a eng
- 044 __
- $a xxk
- 100 1_
- $a Prajzlerová, Klára $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
- 245 14
- $a The dysregulation of monocyte subpopulations in individuals at risk of developing rheumatoid arthritis / $c K. Prajzlerová, O. Kryštůfková, M. Komarc, H. Mann, H. Hulejová, N. Petrovská, M. Gregová, P. Hánová, K. Pavelka, J. Vencovský, L. Šenolt, M. Filková
- 520 9_
- $a OBJECTIVES: Individuals carrying antibodies against citrullinated proteins (ACPA) are at high risk of developing RA. EULAR provided a clinical definition of individuals with arthralgia suspicious for progression to RA (clinically suspect arthralgia, CSA). The alteration of monocyte subpopulations in patients with established RA has been previously described. We analysed peripheral blood monocyte subpopulations in individuals with arthralgia at risk of RA. METHODS: We included 70 at-risk individuals, defined as having arthralgia without arthritis and being either ACPA+ or meeting the clinical CSA definition, 23 patients with early RA (ERA) and 19 healthy controls (HCs). Monocytes classified as classical (CD14++CD16-), intermediate (CD14++CD16+/++) and nonclassical (CD14-/+CD16++) were analysed by flow cytometry. RESULTS: Of the 70 at-risk individuals, 46 were ACPA+ and 45 met the CSA definition. The at-risk individuals and, especially, ERA patients had a lower percentage of classical monocytes and a higher percentage of nonclassical monocytes than the HCs. ACPA positivity had no effect on the difference in the distribution of the monocyte subsets between at-risk individuals and ERA patients, but a difference was determined in those reaching the ERA phase. However, when compared with HCs, the shift of monocyte subsets was more significant in ACPA+ than in ACPA- individuals with arthralgia. This trend was observed in individuals who did not meet the CSA definition. This finding was, however, determined by a selection bias, as these individuals were solely ACPA+. CONCLUSION: The shift from classical to nonclassical monocyte subpopulations was observed already in individuals at risk of developing RA.
- 650 _2
- $a dospělí $7 D000328
- 650 _2
- $a protilátky proti citrulinovaným peptidům $x krev $7 D000075422
- 650 _2
- $a artralgie $x etiologie $7 D018771
- 650 _2
- $a revmatoidní artritida $x krev $x diagnóza $7 D001172
- 650 _2
- $a C-reaktivní protein $x analýza $7 D002097
- 650 _2
- $a studie případů a kontrol $7 D016022
- 650 _2
- $a progrese nemoci $7 D018450
- 650 _2
- $a ženské pohlaví $7 D005260
- 650 _2
- $a následné studie $7 D005500
- 650 _2
- $a lidé $7 D006801
- 650 _2
- $a mužské pohlaví $7 D008297
- 650 _2
- $a lidé středního věku $7 D008875
- 650 _2
- $a monocyty $x metabolismus $7 D009000
- 655 _2
- $a časopisecké články $7 D016428
- 655 _2
- $a práce podpořená grantem $7 D013485
- 700 1_
- $a Kryštůfková, Olga $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
- 700 1_
- $a Komarc, Martin $u Department of Methodology, Faculty of Physical Education and Sport, Charles University, Prague, Czech Republic
- 700 1_
- $a Mann, Heřman $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
- 700 1_
- $a Hulejová, Hana $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic
- 700 1_
- $a Petrovská, Nora $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic $7 xx0264033
- 700 1_
- $a Gregová, Monika $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
- 700 1_
- $a Hánová, Petra $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic
- 700 1_
- $a Pavelka, Karel $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
- 700 1_
- $a Vencovský, Jiří $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
- 700 1_
- $a Šenolt, Ladislav $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
- 700 1_
- $a Filková, Mária $u Department of Experimental Rheumatology, Institute of Rheumatology, Prague, Czech Republic $u Department of Rheumatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
- 773 0_
- $w MED00011379 $t Rheumatology (Oxford, England) $x 1462-0332 $g Roč. 60, č. 4 (2021), s. 1823-1831
- 856 41
- $u https://pubmed.ncbi.nlm.nih.gov/33119082 $y Pubmed
- 910 __
- $a ABA008 $b sig $c sign $y p $z 0
- 990 __
- $a 20210728 $b ABA008
- 991 __
- $a 20210914145614 $b ABA008
- 999 __
- $a ok $b bmc $g 1689834 $s 1139311
- BAS __
- $a 3
- BAS __
- $a PreBMC
- BMC __
- $a 2021 $b 60 $c 4 $d 1823-1831 $e 20210406 $i 1462-0332 $m Rheumatology $n Rheumatology (Oxford) $x MED00011379
- GRA __
- $a NV17-32612A $p MZ0
- LZP __
- $a Pubmed-20210728
