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Matriptase-2 and Hemojuvelin in Hepcidin Regulation: In Vivo Immunoblot Studies in Mask Mice
J. Krijt, J. Frýdlová, I. Gurieva, P. Přikryl, M. Báječný, AU. Steinbicker, M. Vokurka, J. Truksa
Jazyk angličtina Země Švýcarsko
Typ dokumentu časopisecké články
Grantová podpora
18-13103S
Grantová Agentura České Republiky
PROGRES Q26
Univerzita Karlova v Praze
LQ1604 National Sustainability Program II
Ministry of Education, Youth and Sports of CR
CZ.1.05/1.1.00/02.0109
Ministry of Education, Youth and Sports of CR
RVO 86652036
Czech Academy of Sciences
Free Medical Journals od 2000
Freely Accessible Science Journals od 2000
PubMed Central od 2007
Europe PubMed Central od 2007
ProQuest Central od 2000-03-01
Open Access Digital Library od 2000-01-01
Open Access Digital Library od 2007-01-01
Health & Medicine (ProQuest) od 2000-03-01
ROAD: Directory of Open Access Scholarly Resources od 2000
Odkazy
PubMed
33800732
DOI
10.3390/ijms22052650
Knihovny.cz E-zdroje
- MeSH
- dietní železo farmakologie MeSH
- erythropoetin farmakologie MeSH
- GPI-vázané proteiny biosyntéza nedostatek genetika fyziologie MeSH
- hepcidiny biosyntéza genetika MeSH
- inhibitor diferenciace 1 biosyntéza genetika MeSH
- játra metabolismus MeSH
- kostní morfogenetický protein 6 biosyntéza genetika MeSH
- membránové proteiny nedostatek genetika fyziologie MeSH
- myši inbrední C57BL MeSH
- myši knockoutované MeSH
- myši MeSH
- orgánová specificita MeSH
- přetížení železem metabolismus MeSH
- promotorové oblasti (genetika) genetika MeSH
- protein hemochromatózy biosyntéza nedostatek genetika fyziologie MeSH
- proteinové domény MeSH
- regulace genové exprese účinky léků MeSH
- rekombinantní proteiny metabolismus MeSH
- serinové endopeptidasy nedostatek genetika fyziologie MeSH
- slezina metabolismus MeSH
- železo nedostatek MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Matriptase-2, a serine protease expressed in hepatocytes, is a negative regulator of hepcidin expression. The purpose of the study was to investigate the interaction of matriptase-2 with hemojuvelin protein in vivo. Mice lacking the matriptase-2 proteolytic activity (mask mice) display decreased content of hemojuvelin protein. Vice versa, the absence of hemojuvelin results in decreased liver content of matriptase-2, indicating that the two proteins interact. To further characterize the role of matriptase-2, we investigated iron metabolism in mask mice fed experimental diets. Administration of iron-enriched diet increased liver iron stores as well as hepcidin expression. Treatment of iron-overloaded mask mice with erythropoietin increased hemoglobin and hematocrit, indicating that the response to erythropoietin is intact in mask mice. Feeding of an iron-deficient diet to mask mice significantly increased spleen weight as well as the splenic content of erythroferrone and transferrin receptor proteins, indicating stress erythropoiesis. Liver hepcidin expression was decreased; expression of Id1 was not changed. Overall, the results suggest a complex interaction between matriptase-2 and hemojuvelin, and demonstrate that hepcidin can to some extent be regulated even in the absence of matriptase-2 proteolytic activity.
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