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Dinucleoside Polyphosphates as RNA Building Blocks with Pairing Ability in Transcription Initiation
R. Benoni, M. Culka, O. Hudeček, L. Gahurova, H. Cahová
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- bakteriofág T7 enzymologie MeSH
- dinukleosidfosfáty genetika metabolismus MeSH
- DNA řízené RNA-polymerasy genetika metabolismus MeSH
- DNA metabolismus MeSH
- iniciace genetické transkripce * MeSH
- párování bází MeSH
- RNA čepičky genetika MeSH
- RNA genetika metabolismus MeSH
- simulace molekulární dynamiky MeSH
- vazba proteinů MeSH
- virové proteiny genetika metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Dinucleoside polyphosphates (NpnNs) were discovered 50 years ago in all cells. They are often called alarmones, even though the molecular target of the alarm has not yet been identified. Recently, we showed that they serve as noncanonical initiating nucleotides (NCINs) and fulfill the role of 5' RNA caps in Escherichia coli. Here, we present molecular insight into their ability to be used as NCINs by T7 RNA polymerase in the initiation phase of transcription. In general, we observed NpnNs to be equally good substrates as canonical nucleotides for T7 RNA polymerase. Surprisingly, the incorporation of ApnGs boosts the production of RNA 10-fold. This behavior is due to the pairing ability of both purine moieties with the -1 and +1 positions of the antisense DNA strand. Molecular dynamic simulations revealed noncanonical pairing of adenosine with the thymine of the DNA.
Citace poskytuje Crossref.org
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