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An Immunohistochemical Study of the Increase in Antioxidant Capacity of Corneal Epithelial Cells by Molecular Hydrogen, Leading to the Suppression of Alkali-Induced Oxidative Stress
C. Cejka, J. Kossl, V. Holan, JH. Zhang, J. Cejkova
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
Hindawi Publishing Open Access od 2008-01-01
PubMed Central od 2008
Europe PubMed Central od 2008
ProQuest Central od 2014-01-01
Open Access Digital Library od 2008-01-01
Open Access Digital Library od 2008-01-01
Open Access Digital Library od 2009-01-01
Medline Complete (EBSCOhost) od 2011-01-01
Health & Medicine (ProQuest) od 2014-01-01
Odkazy
PubMed
32655773
DOI
10.1155/2020/7435260
Knihovny.cz E-zdroje
- MeSH
- alkálie toxicita MeSH
- antioxidancia metabolismus MeSH
- chemické popálení farmakoterapie metabolismus patologie MeSH
- epitelové buňky účinky léků metabolismus patologie MeSH
- hojení ran účinky léků MeSH
- králíci MeSH
- modely nemocí na zvířatech MeSH
- neovaskularizace rohovky prevence a kontrola MeSH
- oxidační stres účinky léků MeSH
- popálení oka chemicky indukované farmakoterapie metabolismus patologie MeSH
- reaktivní formy kyslíku metabolismus MeSH
- rohovka krevní zásobení účinky léků metabolismus patologie MeSH
- vodík farmakologie terapeutické užití MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Corneal alkali burns are potentially blinding injuries. Alkali induces oxidative stress in corneas followed by excessive corneal inflammation, neovascularization, and untransparent scar formation. Molecular hydrogen (H2), a potent reactive oxygen species (ROS) scavenger, suppresses oxidative stress and enables corneal healing when applied on the corneal surface. The purpose of this study was to examine whether the H2 pretreatment of healthy corneas evokes a protective effect against corneal alkali-induced oxidative stress. Rabbit eyes were pretreated with a H2 solution or buffer solution, by drops onto the ocular surface, and the corneas were then burned with 0.25 M NaOH. The results obtained with immunohistochemistry and pachymetry showed that in the corneas of H2-pretreated eyes, slight oxidative stress appeared followed by an increased expression of antioxidant enzymes. When these corneas were postburned with alkali, the alkali-induced oxidative stress was suppressed. This was in contrast to postburned buffer-pretreated corneas, where the oxidative stress was strong. These corneas healed with scar formation and neovascularization, whereas corneas of H2-pretreated eyes healed with restoration of transparency in the majority of cases. Corneal neovascularization was strongly suppressed. Our results suggest that the corneal alkali-induced oxidative stress was reduced via the increased antioxidant capacity of corneal cells against reactive oxygen species (ROS). It is further suggested that the ability of H2 to induce the increase in antioxidant cell capacity is important for eye protection against various diseases or external influences associated with ROS production.
Faculty of Natural Science Charles University Vinicna 7 12843 Prague 2 Czech Republic
Loma Linda University School of Medicine Loma Linda CA 92350 USA
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