Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Neutrophil Extracellular Trap Induced Dendritic Cell Activation Leads to Th1 Polarization in Type 1 Diabetes

Z. Parackova, I. Zentsova, P. Vrabcova, A. Klocperk, Z. Sumnik, S. Pruhova, L. Petruzelkova, R. Hasler, A. Sediva

. 2020 ; 11 (-) : 661. [pub] 20200414

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21020637

Grantová podpora
NV16-32838A MZ0 CEP - Centrální evidence projektů

Neutrophils releasing neutrophil extracellular traps (NETs) infiltrate the pancreas prior to type 1 diabetes (T1D) onset; however, the precise nature of their contribution to disease remains poorly defined. To examine how NETs affect immune functions in T1D, we investigated NET composition and their effect on dendritic cells (DCs) and T lymphocytes in T1D children. We showed that T1D patient NET composition differs substantially from that of healthy donors and that the presence of T1D-NETs in a mixed peripheral blood mononuclear cell culture caused a strong shift toward IFNγ-producing T lymphocytes, mediated through activation of innate immunity cells in T1D samples. Importantly, in a monocyte-derived DC (moDC) culture, NETs induced cytokine production, phenotypic change and IFNγ-producing T cells only in samples from T1D patients but not in those from healthy donors. RNA-seq analysis revealed that T1D-NETs presence causes TGFβ downregulation and IFNα upregulation and creates pro-T1D signature in healthy moDCs.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21020637
003      
CZ-PrNML
005      
20210830102245.0
007      
ta
008      
210728s2020 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3389/fimmu.2020.00661 $2 doi
035    __
$a (PubMed)32346380
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Parackova, Zuzana $u Department of Immunology, 2nd Faculty of Medicine Charles University, University Hospital in Motol, Prague, Czechia
245    10
$a Neutrophil Extracellular Trap Induced Dendritic Cell Activation Leads to Th1 Polarization in Type 1 Diabetes / $c Z. Parackova, I. Zentsova, P. Vrabcova, A. Klocperk, Z. Sumnik, S. Pruhova, L. Petruzelkova, R. Hasler, A. Sediva
520    9_
$a Neutrophils releasing neutrophil extracellular traps (NETs) infiltrate the pancreas prior to type 1 diabetes (T1D) onset; however, the precise nature of their contribution to disease remains poorly defined. To examine how NETs affect immune functions in T1D, we investigated NET composition and their effect on dendritic cells (DCs) and T lymphocytes in T1D children. We showed that T1D patient NET composition differs substantially from that of healthy donors and that the presence of T1D-NETs in a mixed peripheral blood mononuclear cell culture caused a strong shift toward IFNγ-producing T lymphocytes, mediated through activation of innate immunity cells in T1D samples. Importantly, in a monocyte-derived DC (moDC) culture, NETs induced cytokine production, phenotypic change and IFNγ-producing T cells only in samples from T1D patients but not in those from healthy donors. RNA-seq analysis revealed that T1D-NETs presence causes TGFβ downregulation and IFNα upregulation and creates pro-T1D signature in healthy moDCs.
650    _2
$a mladiství $7 D000293
650    _2
$a dospělí $7 D000328
650    _2
$a buněčná diferenciace $7 D002454
650    _2
$a kultivované buňky $7 D002478
650    _2
$a dítě $7 D002648
650    _2
$a dendritické buňky $x imunologie $7 D003713
650    _2
$a diabetes mellitus 1. typu $x imunologie $7 D003922
650    _2
$a extracelulární pasti $x imunologie $7 D065206
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a regulace genové exprese $7 D005786
650    _2
$a lidé $7 D006801
650    _2
$a přirozená imunita $7 D007113
650    _2
$a interferon gama $x metabolismus $7 D007371
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a neutrofily $x imunologie $7 D009504
650    _2
$a Th1 buňky $x imunologie $7 D018417
650    _2
$a rovnováha Th1-Th2 $7 D058408
650    _2
$a transformující růstový faktor beta $x metabolismus $7 D016212
650    _2
$a mladý dospělý $7 D055815
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Zentsova, Irena $u Department of Immunology, 2nd Faculty of Medicine Charles University, University Hospital in Motol, Prague, Czechia
700    1_
$a Vrabcova, Petra $u Department of Immunology, 2nd Faculty of Medicine Charles University, University Hospital in Motol, Prague, Czechia
700    1_
$a Klocperk, Adam $u Department of Immunology, 2nd Faculty of Medicine Charles University, University Hospital in Motol, Prague, Czechia
700    1_
$a Sumnik, Zdenek $u Department of Pediatrics, 2nd Faculty of Medicine Charles University, University Hospital in Motol, Prague, Czechia
700    1_
$a Pruhova, Stepanka $u Department of Pediatrics, 2nd Faculty of Medicine Charles University, University Hospital in Motol, Prague, Czechia
700    1_
$a Petruzelkova, Lenka $u Department of Pediatrics, 2nd Faculty of Medicine Charles University, University Hospital in Motol, Prague, Czechia
700    1_
$a Hasler, Robert $u Institute of Clinical Molecular Biology, University Hospital in Schleswig-Holstein, Kiel, Germany $u Christian-Albrecht University of Kiel, Kiel, Germany
700    1_
$a Sediva, Anna $u Department of Immunology, 2nd Faculty of Medicine Charles University, University Hospital in Motol, Prague, Czechia
773    0_
$w MED00181405 $t Frontiers in immunology $x 1664-3224 $g Roč. 11, č. - (2020), s. 661
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32346380 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20210728 $b ABA008
991    __
$a 20210830102246 $b ABA008
999    __
$a ok $b bmc $g 1691249 $s 1141083
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 11 $c - $d 661 $e 20200414 $i 1664-3224 $m Frontiers in immunology $n Front Immunol $x MED00181405
GRA    __
$a NV16-32838A $p MZ0
LZP    __
$a Pubmed-20210728
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace