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New planar light source for the induction and monitoring of photodynamic processes in vitro
R. Bajgar, M. Pola, J. Hosik, P. Turjanica, J. Cengery, H. Kolarova
Jazyk angličtina Země Nizozemsko
Typ dokumentu časopisecké články, práce podpořená grantem
NLK
Free Medical Journals
od 2006 do Před 1 rokem
PubMed Central
od 1997 do Před 1 rokem
Europe PubMed Central
od 1997 do Před 1 rokem
ProQuest Central
od 1997-03-01 do Před 1 rokem
Health & Medicine (ProQuest)
od 1997-03-01 do Před 1 rokem
- MeSH
- biologické modely * MeSH
- fibroblasty cytologie metabolismus účinky záření MeSH
- lidé MeSH
- nádorové buněčné linie MeSH
- reaktivní formy kyslíku metabolismus MeSH
- světlo * MeSH
- vysoká teplota MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
We recently developed a new light source that allows for the continuous monitoring of light-induced changes using common spectrophotometric devices adapted for microplate analyses. This source was designed primarily to induce photodynamic processes in cell models. Modern light components, such as LED chips, were used to improve the irradiance homogeneity. In addition, this source forms a small hermetic chamber and thus allows for the regulation of the surrounding atmosphere, which plays a significant role in these light-dependent reactions. The efficacy of the new light source was proven via kinetic measurements of reactive oxygen species generated during the photodynamic reaction of chloroaluminium phthalocyanine disulfonate (ClAlPcS2) in three cell lines: human melanoma cells (G361), human breast adenocarcinoma cells (MCF7), and human fibroblasts (BJ).
Citace poskytuje Crossref.org
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- $a Bajgar, Robert $u Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15, Olomouc, Czech Republic. bajgar@tunw.upol.cz $u Faculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacky University in Olomouc, Hnevotinska 3, 775 15, Olomouc, Czech Republic. bajgar@tunw.upol.cz $7 xx0322708
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- $a We recently developed a new light source that allows for the continuous monitoring of light-induced changes using common spectrophotometric devices adapted for microplate analyses. This source was designed primarily to induce photodynamic processes in cell models. Modern light components, such as LED chips, were used to improve the irradiance homogeneity. In addition, this source forms a small hermetic chamber and thus allows for the regulation of the surrounding atmosphere, which plays a significant role in these light-dependent reactions. The efficacy of the new light source was proven via kinetic measurements of reactive oxygen species generated during the photodynamic reaction of chloroaluminium phthalocyanine disulfonate (ClAlPcS2) in three cell lines: human melanoma cells (G361), human breast adenocarcinoma cells (MCF7), and human fibroblasts (BJ).
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