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New planar light source for the induction and monitoring of photodynamic processes in vitro
R. Bajgar, M. Pola, J. Hosik, P. Turjanica, J. Cengery, H. Kolarova
Language English Country Netherlands
Document type Journal Article, Research Support, Non-U.S. Gov't
NLK
Free Medical Journals
from 2006 to 1 year ago
PubMed Central
from 1997 to 1 year ago
Europe PubMed Central
from 1997 to 1 year ago
ProQuest Central
from 1997-03-01 to 1 year ago
Health & Medicine (ProQuest)
from 1997-03-01 to 1 year ago
- MeSH
- Models, Biological * MeSH
- Fibroblasts cytology metabolism radiation effects MeSH
- Humans MeSH
- Cell Line, Tumor MeSH
- Reactive Oxygen Species metabolism MeSH
- Light * MeSH
- Hot Temperature MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
We recently developed a new light source that allows for the continuous monitoring of light-induced changes using common spectrophotometric devices adapted for microplate analyses. This source was designed primarily to induce photodynamic processes in cell models. Modern light components, such as LED chips, were used to improve the irradiance homogeneity. In addition, this source forms a small hermetic chamber and thus allows for the regulation of the surrounding atmosphere, which plays a significant role in these light-dependent reactions. The efficacy of the new light source was proven via kinetic measurements of reactive oxygen species generated during the photodynamic reaction of chloroaluminium phthalocyanine disulfonate (ClAlPcS2) in three cell lines: human melanoma cells (G361), human breast adenocarcinoma cells (MCF7), and human fibroblasts (BJ).
References provided by Crossref.org
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- $a Bajgar, Robert $u Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University in Olomouc, Hnevotinska 3, 775 15, Olomouc, Czech Republic. bajgar@tunw.upol.cz $u Faculty of Medicine and Dentistry, Institute of Molecular and Translational Medicine, Palacky University in Olomouc, Hnevotinska 3, 775 15, Olomouc, Czech Republic. bajgar@tunw.upol.cz $7 xx0322708
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- $a We recently developed a new light source that allows for the continuous monitoring of light-induced changes using common spectrophotometric devices adapted for microplate analyses. This source was designed primarily to induce photodynamic processes in cell models. Modern light components, such as LED chips, were used to improve the irradiance homogeneity. In addition, this source forms a small hermetic chamber and thus allows for the regulation of the surrounding atmosphere, which plays a significant role in these light-dependent reactions. The efficacy of the new light source was proven via kinetic measurements of reactive oxygen species generated during the photodynamic reaction of chloroaluminium phthalocyanine disulfonate (ClAlPcS2) in three cell lines: human melanoma cells (G361), human breast adenocarcinoma cells (MCF7), and human fibroblasts (BJ).
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- $a Turjanica, P $u Regional Innovation Centre for Electrical Engineering, University of West Bohemia, Univerzitni 26, 306 14, Pilsen, Czech Republic
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