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Smart co-delivery of miR-34a and cytotoxic peptides (LTX-315 and melittin) by chitosan based polyelectrolyte nanocarriers for specific cancer cell death induction
M. Motiei, F. Aboutalebi, M. Forouzanfar, K. Dormiani, MH. Nasr-Esfahani, SZ. Mirahmadi-Zare
Language English Country Netherlands
Document type Journal Article
- MeSH
- Cell Death MeSH
- Chitosan * MeSH
- Humans MeSH
- Melitten pharmacology MeSH
- MicroRNAs administration & dosage genetics MeSH
- Cell Line, Tumor MeSH
- Breast Neoplasms * drug therapy MeSH
- Nanoparticles * MeSH
- Oligopeptides MeSH
- Polyelectrolytes MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Publication type
- Journal Article MeSH
A novel polyelectrolyte nanocarrier was synthesized via layer-by-layer self-assembly of polycationic and polyanionic chains. The nanocarrier is composed of polyglutamate grafted chitosan core, dextran sulfate as a complexing agent, and polyethyleneimine shell decorated with folic acid. This polyelectrolyte complex has unique physicochemical properties so that the core is considered as an efficient carrier for LTX-315 and melittin peptides, and the shell is suitable for delivery of miR-34a. The spherical nanocarriers with an average size of 123 ± 5 nm and a zeta potential of -36 ± 1 mV demonstrated controlled-release of gene and peptides ensured a synergistic effect in establishing multiple cell death pathways on chemoresistance human breast adenocarcinoma cell line, MDA-MB-231. In vitro cell viability assays also revealed no cytotoxicity for the nanocarriers, and an IC50 of 15 μg/mL and 150 μg/mL for melittin and LTX-315, respectively, after 48 h, whereas co-delivery of melittin with miR-34a increased smart death induction by 54%.
References provided by Crossref.org
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