Detail
Článek
Článek online
FT
Medvik - BMČ
  • Je něco špatně v tomto záznamu ?

Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells

J. Hofmanová, J. Slavík, M. Ciganek, P. Ovesná, Z. Tylichová, M. Karasová, O. Zapletal, N. Straková, J. Procházková, J. Bouchal, Z. Kolář, J. Ehrmann, M. Levková, Z. Hušková, P. Skalický, A. Kozubík, M. Machala, J. Vondráček

. 2021 ; 22 (13) : . [pub] 20210622

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21025547

Grantová podpora
15-30585A Ministerstvo Zdravotnictví Ceské Republiky
RVO: 68081707 Akademie Věd České Republiky
DRO-FNOL00098892 Ministerstvo Zdravotnictví Ceské Republiky
RO0520 Ministerstvo Zemědělství
CZ.02.1.01/0.0/0.0/15_003/0000495 Ministerstvo Školství, Mládeže a Tělovýchovy
CZ.02.1.01/0.0/0.0/16_019/0000868 Ministerstvo Školství, Mládeže a Tělovýchovy
CZ.02.1.01/0.0/0.0/16_013/0001674 Ministerstvo Školství, Mládeže a Tělovýchovy

The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM+ cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21025547
003      
CZ-PrNML
005      
20230119101112.0
007      
ta
008      
211013s2021 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/ijms22136650 $2 doi
035    __
$a (PubMed)34206240
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Hofmanová, Jiřina $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 612 65 Brno, Czech Republic $u Department of Experimental Biology, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic
245    10
$a Complex Alterations of Fatty Acid Metabolism and Phospholipidome Uncovered in Isolated Colon Cancer Epithelial Cells / $c J. Hofmanová, J. Slavík, M. Ciganek, P. Ovesná, Z. Tylichová, M. Karasová, O. Zapletal, N. Straková, J. Procházková, J. Bouchal, Z. Kolář, J. Ehrmann, M. Levková, Z. Hušková, P. Skalický, A. Kozubík, M. Machala, J. Vondráček
520    9_
$a The development of colon cancer, one of the most common malignancies, is accompanied with numerous lipid alterations. However, analyses of whole tumor samples may not always provide an accurate description of specific changes occurring directly in tumor epithelial cells. Here, we analyzed in detail the phospholipid (PL), lysophospholipid (lysoPL), and fatty acid (FA) profiles of purified EpCAM+ cells, isolated from tumor and adjacent non-tumor tissues of colon cancer patients. We found that a number of FAs increased significantly in isolated tumor cells, which also included a number of long polyunsaturated FAs. Higher levels of FAs were associated with increased expression of FA synthesis genes, as well as with altered expression of enzymes involved in FA elongation and desaturation, including particularly fatty acid synthase, stearoyl-CoA desaturase, fatty acid desaturase 2 and ELOVL5 fatty acid elongase 5 We identified significant changes in ratios of specific lysoPLs and corresponding PLs. A number of lysophosphatidylcholine and lysophosphatidylethanolamine species, containing long-chain and very-long chain FAs, often with high numbers of double bonds, were significantly upregulated in tumor cells. Increased de novo synthesis of very long-chain FAs, or, altered uptake or incorporation of these FAs into specific lysoPLs in tumor cells, may thus contribute to reprogramming of cellular phospholipidome and membrane alterations observed in colon cancer.
650    _2
$a adenokarcinom $x enzymologie $x genetika $x metabolismus $7 D000230
650    _2
$a senioři $7 D000368
650    _2
$a nádory tračníku $x enzymologie $x genetika $x metabolismus $7 D003110
650    _2
$a epitelové buňky $x enzymologie $x metabolismus $7 D004847
650    _2
$a desaturasy mastných kyselin $x genetika $x metabolismus $7 D044943
650    _2
$a elongasy mastných kyselin $x genetika $x metabolismus $7 D000081031
650    _2
$a syntázy mastných kyselin $x genetika $x metabolismus $7 D064429
650    _2
$a mastné kyseliny $x metabolismus $7 D005227
650    _2
$a ženské pohlaví $7 D005260
650    12
$a regulace genové exprese u nádorů $7 D015972
650    _2
$a lidé $7 D006801
650    12
$a metabolismus lipidů $7 D050356
650    _2
$a lipidomika $7 D000081362
650    _2
$a lipogeneze $7 D050155
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a fosfolipidy $x metabolismus $7 D010743
650    _2
$a stearyl-CoA-desaturasa $x genetika $x metabolismus $7 D013230
655    _2
$a časopisecké články $7 D016428
700    1_
$a Slavík, Josef $u Department of Pharmacology and Toxicology, Veterinary Research Institute, 621 00 Brno, Czech Republic
700    1_
$a Ciganek, Miroslav $u Department of Pharmacology and Toxicology, Veterinary Research Institute, 621 00 Brno, Czech Republic
700    1_
$a Ovesná, Petra $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 612 65 Brno, Czech Republic $u Institute of Biostatistics and Analyses, Faculty of Medicine, Masaryk University, 602 00 Brno, Czech Republic
700    1_
$a Tylichová, Zuzana $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 612 65 Brno, Czech Republic
700    1_
$a Karasová, Martina $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 612 65 Brno, Czech Republic $u Department of Experimental Biology, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic
700    1_
$a Zapletal, Ondřej $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 612 65 Brno, Czech Republic
700    1_
$a Straková, Nicol $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 612 65 Brno, Czech Republic $u Department of Pharmacology and Toxicology, Veterinary Research Institute, 621 00 Brno, Czech Republic
700    1_
$a Procházková, Jiřina $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 612 65 Brno, Czech Republic $u Department of Pharmacology and Toxicology, Veterinary Research Institute, 621 00 Brno, Czech Republic
700    1_
$a Bouchal, Jan $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacký University and University Hospital, 779 00 Olomouc, Czech Republic
700    1_
$a Kolář, Zdeněk $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacký University and University Hospital, 779 00 Olomouc, Czech Republic
700    1_
$a Ehrmann, Jiří $u Department of Surgery, University Hospital, 779 00 Olomouc, Czech Republic
700    1_
$a Levková, Monika $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacký University and University Hospital, 779 00 Olomouc, Czech Republic $7 xx0280839
700    1_
$a Hušková, Zlatka $u Department of Clinical and Molecular Pathology, Institute of Molecular and Translational Medicine, Faculty of Medicine, Palacký University and University Hospital, 779 00 Olomouc, Czech Republic
700    1_
$a Skalický, Pavel $u Department of Surgery, University Hospital, 779 00 Olomouc, Czech Republic
700    1_
$a Kozubík, Alois $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 612 65 Brno, Czech Republic $u Department of Experimental Biology, Faculty of Science, Masaryk University, 625 00 Brno, Czech Republic
700    1_
$a Machala, Miroslav $u Department of Pharmacology and Toxicology, Veterinary Research Institute, 621 00 Brno, Czech Republic
700    1_
$a Vondráček, Jan $u Department of Cytokinetics, Institute of Biophysics of the Czech Academy of Sciences, 612 65 Brno, Czech Republic
773    0_
$w MED00176142 $t International journal of molecular sciences $x 1422-0067 $g Roč. 22, č. 13 (2021)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34206240 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211013 $b ABA008
991    __
$a 20230119101105 $b ABA008
999    __
$a ok $b bmc $g 1714552 $s 1146054
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 22 $c 13 $e 20210622 $i 1422-0067 $m International journal of molecular sciences $n Int J Mol Sci $x MED00176142
GRA    __
$a 15-30585A $p Ministerstvo Zdravotnictví Ceské Republiky
GRA    __
$a RVO: 68081707 $p Akademie Věd České Republiky
GRA    __
$a DRO-FNOL00098892 $p Ministerstvo Zdravotnictví Ceské Republiky
GRA    __
$a RO0520 $p Ministerstvo Zemědělství
GRA    __
$a CZ.02.1.01/0.0/0.0/15_003/0000495 $p Ministerstvo Školství, Mládeže a Tělovýchovy
GRA    __
$a CZ.02.1.01/0.0/0.0/16_019/0000868 $p Ministerstvo Školství, Mládeže a Tělovýchovy
GRA    __
$a CZ.02.1.01/0.0/0.0/16_013/0001674 $p Ministerstvo Školství, Mládeže a Tělovýchovy
LZP    __
$a Pubmed-20211013

Najít záznam

Citační ukazatele

Možnosti archivace