The Arabidopsis non-specific phospholipase C (NPC) protein family is encoded by the genes NPC1 - NPC6. It has been shown that NPC4 and NPC5 possess phospholipase C activity; NPC3 has lysophosphatidic acid phosphatase activity. NPC3, 4 and 5 play roles in the responses to hormones and abiotic stresses. NPC1, 2 and 6 has not been studied functionally yet. We found that Arabidopsis NPC1 expressed in Escherichia coli possesses phospholipase C activity in vitro. This protein was able to hydrolyse phosphatidylcholine to diacylglycerol. NPC1-green fluorescent protein was localized to secretory pathway compartments in Arabidopsis roots. In the knock out T-DNA insertion line NPC1 (npc1) basal thermotolerance was impaired compared with wild-type (WT); npc1 exhibited significant decreases in survival rate and chlorophyll content at the seventh day after heat stress (HS). Conversely, plants overexpressing NPC1 (NPC1-OE) were more resistant to HS compared with WT. These findings suggest that NPC1 is involved in the plant response to heat.

CNRS UMR7618 Institut d'Ecologie et des Sciences de l'Environnement de Paris Créteil France ; Université Paris Est Institut d'Ecologie et des Sciences de l'Environnement de Paris UPEC Créteil France

Department of Biochemistry and Microbiology University of Chemistry and Technology Prague Czech Republic

ERL Inserm U1157 UMR7203 Faculté de Medecine Pierre et Marie Curie Paris France

Institute of Experimental Botany The Czech Academy of Sciences Prague Czech Republic

Institute of Experimental Botany The Czech Academy of Sciences Prague Czech Republic ; Department of Biochemistry and Microbiology University of Chemistry and Technology Prague Czech Republic

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Recent Advances in the Cellular and Developmental Biology of Phospholipases in Plants

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