Targeting of the heat stress (HS, 40°C) to shoots, roots or whole plants substantially affects Arabidopsis physiological responses. Effective stress targeting was proved by determination of the expression of HS markers, HsfA2 and HSA32, which were quickly stimulated in the targeted organ(s), but remained low in non-stressed tissues for at least 2h. When shoots or whole plants were subjected to HS, a transient decrease in abscisic acid, accompanied by a small increase in active cytokinin levels, was observed in leaves, consistent with stimulation of transpiration, the main cooling mechanism in leaves. HS application targeted to part of plant resulted in a rapid stimulation of expression of components of cytokinin signaling pathway (especially of receptor genes) in the non-exposed tissues, which indicated fast inter-organ communication. Under all HS treatments, shoot apices responded by transient elevation of active cytokinin contents and stimulation of transcription of genes involved in photosynthesis and carbohydrate metabolism. Duration of this stimulation was negatively correlated with stress strength. The impact of targeted HS on the expression of 63 selected genes, including those coding regulatory 14-3-3 proteins, was compared. Stimulation of GRF9 (GRF14μ) in stressed organs after 2-6h may be associated with plant stress adaptation.

Faculty of Science Charles University Viničná 7 CZ 12843 Prague Czech Republic

Institute of Experimental Botany AS CR Rozvojová 263 CZ 16502 Prague 6 Czech Republic

Laboratory of Plant Molecular Biology Institute of Biophysics AS CR v v i and CEITEC Central European Institute of Technology Mendel University in Brno CZ 61300 Brno Czech Republic

Citace poskytuje Crossref.org

Crosstalk between Light- and Temperature-Mediated Processes under Cold and Heat Stress Conditions in Plants

Light Quality and Intensity Modulate Cold Acclimation in Arabidopsis

Priming Maritime Pine Megagametophytes during Somatic Embryogenesis Improved Plant Adaptation to Heat Stress

Signal Integration in Plant Abiotic Stress Responses via Multistep Phosphorelay Signaling

Heat Stress Targeting Individual Organs Reveals the Central Role of Roots and Crowns in Rice Stress Responses

Arabidopsis Response to Inhibitor of Cytokinin Degradation INCYDE: Modulations of Cytokinin Signaling and Plant Proteome

Heat Acclimation and Inhibition of Cytokinin Degradation Positively Affect Heat Stress Tolerance of Arabidopsis

Light Regulates the Cytokinin-Dependent Cold Stress Responses in Arabidopsis

Cytokinin at the Crossroads of Abiotic Stress Signalling Pathways

Stimulation of ipt overexpression as a tool to elucidate the role of cytokinins in high temperature responses of Arabidopsis thaliana

Arabidopsis non-specific phospholipase C1: characterization and its involvement in response to heat stress