• Je něco špatně v tomto záznamu ?

Honey Phenolic Compound Profiling and Authenticity Assessment Using HRMS Targeted and Untargeted Metabolomics

GA. Koulis, AS. Tsagkaris, R. Aalizadeh, ME. Dasenaki, EI. Panagopoulou, S. Drivelos, M. Halagarda, CA. Georgiou, C. Proestos, NS. Thomaidis

. 2021 ; 26 (9) : . [pub] 20210508

Jazyk angličtina Země Švýcarsko

Typ dokumentu časopisecké články, validační studie

Perzistentní odkaz   https://www.medvik.cz/link/bmc21025749

Grantová podpora
2084 Hellenic Foundation for Research and Innovation

Honey consumption is attributed to potentially advantageous effects on human health due to its antioxidant capacity as well as anti-inflammatory and antimicrobial activity, which are mainly related to phenolic compound content. Phenolic compounds are secondary metabolites of plants, and their content in honey is primarily affected by the botanical and geographical origin. In this study, a high-resolution mass spectrometry (HRMS) method was applied to determine the phenolic profile of various honey matrices and investigate authenticity markers. A fruitful sample set was collected, including honey from 10 different botanical sources (n = 51) originating from Greece and Poland. Generic liquid-liquid extraction using ethyl acetate as the extractant was used to apply targeted and non-targeted workflows simultaneously. The method was fully validated according to the Eurachem guidelines, and it demonstrated high accuracy, precision, and sensitivity resulting in the detection of 11 target analytes in the samples. Suspect screening identified 16 bioactive compounds in at least one sample, with abscisic acid isomers being the most abundant in arbutus honey. Importantly, 10 markers related to honey geographical origin were revealed through non-targeted screening and the application of advanced chemometric tools. In conclusion, authenticity markers and discrimination patterns were emerged using targeted and non-targeted workflows, indicating the impact of this study on food authenticity and metabolomic fields.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21025749
003      
CZ-PrNML
005      
20211026133539.0
007      
ta
008      
211013s2021 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3390/molecules26092769 $2 doi
035    __
$a (PubMed)34066694
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Koulis, Georgios A $u Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece $u Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece
245    10
$a Honey Phenolic Compound Profiling and Authenticity Assessment Using HRMS Targeted and Untargeted Metabolomics / $c GA. Koulis, AS. Tsagkaris, R. Aalizadeh, ME. Dasenaki, EI. Panagopoulou, S. Drivelos, M. Halagarda, CA. Georgiou, C. Proestos, NS. Thomaidis
520    9_
$a Honey consumption is attributed to potentially advantageous effects on human health due to its antioxidant capacity as well as anti-inflammatory and antimicrobial activity, which are mainly related to phenolic compound content. Phenolic compounds are secondary metabolites of plants, and their content in honey is primarily affected by the botanical and geographical origin. In this study, a high-resolution mass spectrometry (HRMS) method was applied to determine the phenolic profile of various honey matrices and investigate authenticity markers. A fruitful sample set was collected, including honey from 10 different botanical sources (n = 51) originating from Greece and Poland. Generic liquid-liquid extraction using ethyl acetate as the extractant was used to apply targeted and non-targeted workflows simultaneously. The method was fully validated according to the Eurachem guidelines, and it demonstrated high accuracy, precision, and sensitivity resulting in the detection of 11 target analytes in the samples. Suspect screening identified 16 bioactive compounds in at least one sample, with abscisic acid isomers being the most abundant in arbutus honey. Importantly, 10 markers related to honey geographical origin were revealed through non-targeted screening and the application of advanced chemometric tools. In conclusion, authenticity markers and discrimination patterns were emerged using targeted and non-targeted workflows, indicating the impact of this study on food authenticity and metabolomic fields.
650    _2
$a antioxidancia $x analýza $x izolace a purifikace $7 D000975
650    _2
$a benzaldehydy $x analýza $x izolace a purifikace $7 D001547
650    _2
$a cinnamáty $x analýza $x izolace a purifikace $7 D002934
650    _2
$a správnost dat $7 D000068598
650    _2
$a flavonoidy $x analýza $x izolace a purifikace $7 D005419
650    _2
$a med $x analýza $7 D006722
650    _2
$a lidé $7 D006801
650    _2
$a hydroxybenzoáty $x analýza $x izolace a purifikace $7 D062385
650    _2
$a hmotnostní spektrometrie $x metody $7 D013058
650    12
$a metabolom $7 D055442
650    _2
$a metabolomika $x metody $7 D055432
650    _2
$a senzitivita a specificita $7 D012680
651    _2
$a Řecko $7 D006115
651    _2
$a Polsko $7 D011044
655    _2
$a časopisecké články $7 D016428
655    _2
$a validační studie $7 D023361
700    1_
$a Tsagkaris, Aristeidis S $u Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece $u Department of Food Analysis and Nutrition, Faculty of Food and Biochemical Technology, University of Chemistry and Technology Prague, Technická 5, Prague 6-Dejvice, 16628 Prague, Czech Republic
700    1_
$a Aalizadeh, Reza $u Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece
700    1_
$a Dasenaki, Marilena E $u Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece $u Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece
700    1_
$a Panagopoulou, Eleni I $u Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece
700    1_
$a Drivelos, Spyros $u Chemistry Laboratory, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece
700    1_
$a Halagarda, Michał $u Department of Food Product Quality, Cracow University of Economics, ul. Sienkiewicza 5, 30033 Krakow, Poland
700    1_
$a Georgiou, Constantinos A $u Chemistry Laboratory, Department of Food Science and Human Nutrition, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece
700    1_
$a Proestos, Charalampos $u Food Chemistry Laboratory, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece
700    1_
$a Thomaidis, Nikolaos S $u Analytical Chemistry Laboratory, Chemistry Department, National and Kapodistrian University of Athens, Panepistimiopolis Zographou, 15771 Athens, Greece
773    0_
$w MED00180394 $t Molecules (Basel, Switzerland) $x 1420-3049 $g Roč. 26, č. 9 (2021)
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34066694 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211013 $b ABA008
991    __
$a 20211026133545 $b ABA008
999    __
$a ok $b bmc $g 1714678 $s 1146256
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 26 $c 9 $e 20210508 $i 1420-3049 $m Molecules $n Molecules $x MED00180394
GRA    __
$a 2084 $p Hellenic Foundation for Research and Innovation
LZP    __
$a Pubmed-20211013

Najít záznam

Citační ukazatele

Možnosti archivace