• Je něco špatně v tomto záznamu ?

Implication of TRPC3 channel in gustatory perception of dietary lipids

B. Murtaza, A. Hichami, AS. Khan, J. Plesnik, O. Sery, A. Dietrich, L. Birnbaumer, NA. Khan

. 2021 ; 231 (2) : e13554. [pub] 20200913

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21026092

AIM: The pathogenesis of obesity has been associated with high intake of dietary fat, and some recent studies have explored the cellular mechanisms of oro-sensory detection of dietary fatty acids. We further assessed the role of transient receptor potential canonical (TRPC) channels in oro-sensory perception of dietary lipids. METHODS: We determined by RT-qPCR and western blotting the expression of TRPC3/6/7 channels in mouse fungiform taste bud cells (mTBC). Immunocytochemistry was used to explore whether TRPC3 channels were co-expressed with fatty acid receptors. We employed wild-type (WT) mTBC, and those transfected with small interfering RNAs (siRNAs) against TRPC3 or STIM1. Ca2+ signalling was studied in TBC from TRPC3-/- mice and their WT littermates. RESULTS: We demonstrate that mouse fungiform taste bud cells (mTBC) express TRPC3, but not TRPC6 or TRPC7 channels, and their inactivation by siRNA or experiments on TBC from TRPC3-/- mice brought about a decrease in fatty acid-induced gustatory Ca2+ signalling, coupled with taste bud CD36 lipid sensor. TRPC3 channel activation was found to be under the control of STIM1 in lingual mTBC. Behavioural studies showed that spontaneous preference for a dietary long-chain fatty acid was abolished in TRPC3-/- mice, and in mice wherein lingual TRPC3 expression was silenced by employing siRNA. CONCLUSION: We report that lingual TRPC3 channels are critically involved in fat taste perception.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21026092
003      
CZ-PrNML
005      
20230125085138.0
007      
ta
008      
211013s2021 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1111/apha.13554 $2 doi
035    __
$a (PubMed)32882106
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Murtaza, Babar $u U1231 INSERM/UB/AgroSup, Physiologie de la Nutrition & Toxicologie, Université de Bourgogne-Franche Comté (UBFC), Dijon, France
245    10
$a Implication of TRPC3 channel in gustatory perception of dietary lipids / $c B. Murtaza, A. Hichami, AS. Khan, J. Plesnik, O. Sery, A. Dietrich, L. Birnbaumer, NA. Khan
520    9_
$a AIM: The pathogenesis of obesity has been associated with high intake of dietary fat, and some recent studies have explored the cellular mechanisms of oro-sensory detection of dietary fatty acids. We further assessed the role of transient receptor potential canonical (TRPC) channels in oro-sensory perception of dietary lipids. METHODS: We determined by RT-qPCR and western blotting the expression of TRPC3/6/7 channels in mouse fungiform taste bud cells (mTBC). Immunocytochemistry was used to explore whether TRPC3 channels were co-expressed with fatty acid receptors. We employed wild-type (WT) mTBC, and those transfected with small interfering RNAs (siRNAs) against TRPC3 or STIM1. Ca2+ signalling was studied in TBC from TRPC3-/- mice and their WT littermates. RESULTS: We demonstrate that mouse fungiform taste bud cells (mTBC) express TRPC3, but not TRPC6 or TRPC7 channels, and their inactivation by siRNA or experiments on TBC from TRPC3-/- mice brought about a decrease in fatty acid-induced gustatory Ca2+ signalling, coupled with taste bud CD36 lipid sensor. TRPC3 channel activation was found to be under the control of STIM1 in lingual mTBC. Behavioural studies showed that spontaneous preference for a dietary long-chain fatty acid was abolished in TRPC3-/- mice, and in mice wherein lingual TRPC3 expression was silenced by employing siRNA. CONCLUSION: We report that lingual TRPC3 channels are critically involved in fat taste perception.
650    _2
$a zvířata $7 D000818
650    _2
$a dietní tuky $7 D004041
650    12
$a preference v jídle $7 D005518
650    _2
$a lipidy $7 D008055
650    _2
$a myši $7 D051379
650    _2
$a kationtové kanály TRPC $x genetika $7 D050052
650    12
$a chuťová percepce $7 D055697
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Hichami, Aziz $u U1231 INSERM/UB/AgroSup, Physiologie de la Nutrition & Toxicologie, Université de Bourgogne-Franche Comté (UBFC), Dijon, France
700    1_
$a Khan, Amira S $u U1231 INSERM/UB/AgroSup, Physiologie de la Nutrition & Toxicologie, Université de Bourgogne-Franche Comté (UBFC), Dijon, France
700    1_
$a Plesnik, Jiri $u U1231 INSERM/UB/AgroSup, Physiologie de la Nutrition & Toxicologie, Université de Bourgogne-Franche Comté (UBFC), Dijon, France $u Laboratory of Neurobiology and Molecular Psychiatry, Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
700    1_
$a Sery, Omar $u Laboratory of Neurobiology and Molecular Psychiatry, Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic $u Laboratory of Neurobiology and Pathological Physiology, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
700    1_
$a Dietrich, Alexander $u Walther-Straub-Institute of Pharmacology and Toxicology, Member of the German Center for Lung Research (DZL), LMU Munich, Munich, Germany
700    1_
$a Birnbaumer, Lutz, $u Neurobiology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, NC, USA $u Institute of Biomedical Research (BIOMED), Catholic University of Argentina, Buenos Aires, Argentina $d 1939- $7 xx0281067
700    1_
$a Khan, Naim A $u U1231 INSERM/UB/AgroSup, Physiologie de la Nutrition & Toxicologie, Université de Bourgogne-Franche Comté (UBFC), Dijon, France
773    0_
$w MED00154561 $t Acta physiologica $x 1748-1716 $g Roč. 231, č. 2 (2021), s. e13554
856    41
$u https://pubmed.ncbi.nlm.nih.gov/32882106 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211013 $b ABA008
991    __
$a 20230125085133 $b ABA008
999    __
$a ok $b bmc $g 1714951 $s 1146599
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 231 $c 2 $d e13554 $e 20200913 $i 1748-1716 $m Acta physiologica $n Acta Physiol $x MED00154561
LZP    __
$a Pubmed-20211013
Zpět

Najít záznam

Citační ukazatele

Možnosti archivace