• Je něco špatně v tomto záznamu ?

N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease

E. Lattová, J. Skřičková, J. Hausnerová, L. Frola, L. Křen, I. Ihnatová, Z. Zdráhal, J. Bryant, M. Popovič

. 2020 ; 33 (6) : 1146-1156. [pub] 20200106

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc21026697

Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age. Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood. In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC. Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease. The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors. In addition, 42 new N-glycan compositions were detected in cancerous tissues. The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units. Both of these monosaccharide types were linked preferably on the 6-antenna. Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy. In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies. Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21026697
003      
CZ-PrNML
005      
20211026132705.0
007      
ta
008      
211013s2020 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41379-019-0441-3 $2 doi
035    __
$a (PubMed)31907375
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Lattová, Erika $u Central European Institute for Technology, Masaryk University, Kamenice 5, Brno, Czech Republic. erika.lattova@gmail.com
245    10
$a N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease / $c E. Lattová, J. Skřičková, J. Hausnerová, L. Frola, L. Křen, I. Ihnatová, Z. Zdráhal, J. Bryant, M. Popovič
520    9_
$a Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age. Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood. In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC. Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease. The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal2GlcNAc2Man3GlcNAc2Fuc were general features of tumors. In addition, 42 new N-glycan compositions were detected in cancerous tissues. The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units. Both of these monosaccharide types were linked preferably on the 6-antenna. Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy. In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies. Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.
650    _2
$a adenokarcinom plic $x metabolismus $x patologie $7 D000077192
650    _2
$a dospělí $7 D000328
650    _2
$a senioři $7 D000368
650    _2
$a progrese nemoci $7 D018450
650    _2
$a ženské pohlaví $7 D005260
650    _2
$a glykosylace $7 D006031
650    _2
$a lidé $7 D006801
650    _2
$a nádory plic $x metabolismus $x patologie $7 D008175
650    _2
$a mužské pohlaví $7 D008297
650    _2
$a lidé středního věku $7 D008875
650    _2
$a polysacharidy $x metabolismus $7 D011134
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Skřičková, Jana $u Department of Respiratory Diseases and TB, University Hospital Brno and Medical Faculty of Masaryk University, Brno, Czech Republic
700    1_
$a Hausnerová, Jitka $u Department of Pathology, University Hospital and Medical Faculty of Masaryk University, Brno, Czech Republic. Hausnerova.Jitka@fnbrno.cz
700    1_
$a Frola, Lukáš $u Department of Pathology, University Hospital and Medical Faculty of Masaryk University, Brno, Czech Republic
700    1_
$a Křen, Leoš $u Department of Pathology, University Hospital and Medical Faculty of Masaryk University, Brno, Czech Republic
700    1_
$a Ihnatová, Ivana $u RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic
700    1_
$a Zdráhal, Zbyněk $u Central European Institute for Technology, Masaryk University, Kamenice 5, Brno, Czech Republic $u National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic
700    1_
$a Bryant, Joseph $u The Institute of Human Virology, University of Maryland, 725W. Lombard St., Baltimore, MD, 21201, USA
700    1_
$a Popovič, Mikuláš $u The Institute of Human Virology, University of Maryland, 725W. Lombard St., Baltimore, MD, 21201, USA
773    0_
$w MED00003380 $t Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc $x 1530-0285 $g Roč. 33, č. 6 (2020), s. 1146-1156
856    41
$u https://pubmed.ncbi.nlm.nih.gov/31907375 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211013 $b ABA008
991    __
$a 20211026132711 $b ABA008
999    __
$a ok $b bmc $g 1715437 $s 1147204
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2020 $b 33 $c 6 $d 1146-1156 $e 20200106 $i 1530-0285 $m Modern pathology $n Mod Pathol $x MED00003380
LZP    __
$a Pubmed-20211013

Najít záznam

Citační ukazatele

Možnosti archivace