• Je něco špatně v tomto záznamu ?

Characterization of a salt-resistant fibrinolytic protease of Bacillus licheniformis HJ4 isolated from Hwangseokae jeotgal, a traditional Korean fermented seafood

Y. Meng, Z. Yao, HG. Le, SJ. Lee, HS. Jeon, JY. Yoo, JH. Kim

. 2021 ; 66 (5) : 787-795. [pub] 20210614

Jazyk angličtina Země Spojené státy americké

Typ dokumentu časopisecké články

Perzistentní odkaz   https://www.medvik.cz/link/bmc21029478

Grantová podpora
2017R1D1A1B03030037 Ministry of Education

Bacillus licheniformis HJ4 showing strong fibrinolytic activity was isolated from Hwangseokae jeotgal. aprEHJ4, a major fibrinolytic gene, was cloned by PCR, and an ORF consisting of 379 amino acids was located. The mature enzyme was expected to be 27 kDa in size after processing, but a 24-kDa protein was observed by SDS-PAGE and fibrin zymography, indicating additional processing. RT-qPCR showed that expression level of aprEHJ4 in culture with 0% salt (control) was the highest followed by culture with 8% salt (89.7% of control) and 5% salt (74.2%) at 84 h. The expression level in culture with 15% salt was 46.9%. The results matched with the fibrinolytic activity measurements of cultures and indicated that AprEHJ4 maintained significant activity in the presence of salt up to 15% (w/v). AprEHJ4 was overproduced in Escherichia coli, and mature 27 kDa protein was purified after in vitro renaturation. The optimum pH and temperature of AprEHJ4 were pH 8 and 40 ℃, respectively.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc21029478
003      
CZ-PrNML
005      
20211206103719.0
007      
ta
008      
211206s2021 xxu f 000 0|eng||
009      
AR
024    7_
$a 10.1007/s12223-021-00878-w $2 doi
035    __
$a (PubMed)34128186
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxu
100    1_
$a Meng, Yu $u Division of Applied Life Science (BK21 Four), Graduate School, Gyeongsang National University, Jinju, 52828, Korea
245    10
$a Characterization of a salt-resistant fibrinolytic protease of Bacillus licheniformis HJ4 isolated from Hwangseokae jeotgal, a traditional Korean fermented seafood / $c Y. Meng, Z. Yao, HG. Le, SJ. Lee, HS. Jeon, JY. Yoo, JH. Kim
520    9_
$a Bacillus licheniformis HJ4 showing strong fibrinolytic activity was isolated from Hwangseokae jeotgal. aprEHJ4, a major fibrinolytic gene, was cloned by PCR, and an ORF consisting of 379 amino acids was located. The mature enzyme was expected to be 27 kDa in size after processing, but a 24-kDa protein was observed by SDS-PAGE and fibrin zymography, indicating additional processing. RT-qPCR showed that expression level of aprEHJ4 in culture with 0% salt (control) was the highest followed by culture with 8% salt (89.7% of control) and 5% salt (74.2%) at 84 h. The expression level in culture with 15% salt was 46.9%. The results matched with the fibrinolytic activity measurements of cultures and indicated that AprEHJ4 maintained significant activity in the presence of salt up to 15% (w/v). AprEHJ4 was overproduced in Escherichia coli, and mature 27 kDa protein was purified after in vitro renaturation. The optimum pH and temperature of AprEHJ4 were pH 8 and 40 ℃, respectively.
650    12
$a Bacillus licheniformis $x enzymologie $7 D000069976
650    _2
$a aktivace enzymů $x účinky léků $7 D004789
650    12
$a fermentované potraviny $x mikrobiologie $7 D000074421
650    _2
$a koncentrace vodíkových iontů $7 D006863
650    _2
$a proteasy $x metabolismus $7 D010447
650    12
$a potrava z moře (živočišná) $x mikrobiologie $7 D017747
650    _2
$a chlorid sodný $x farmakologie $7 D012965
651    _2
$a Korejská republika $7 D056910
655    _2
$a časopisecké články $7 D016428
700    1_
$a Yao, Zhuang $u Division of Applied Life Science (BK21 Four), Graduate School, Gyeongsang National University, Jinju, 52828, Korea
700    1_
$a Le, Huong Giang $u Division of Applied Life Science (BK21 Four), Graduate School, Gyeongsang National University, Jinju, 52828, Korea
700    1_
$a Lee, Se Jin $u Division of Applied Life Science (BK21 Four), Graduate School, Gyeongsang National University, Jinju, 52828, Korea
700    1_
$a Jeon, Hye Sung $u Division of Applied Life Science (BK21 Four), Graduate School, Gyeongsang National University, Jinju, 52828, Korea
700    1_
$a Yoo, Ji Yeon $u Division of Applied Life Science (BK21 Four), Graduate School, Gyeongsang National University, Jinju, 52828, Korea
700    1_
$a Kim, Jeong Hwan $u Division of Applied Life Science (BK21 Four), Graduate School, Gyeongsang National University, Jinju, 52828, Korea. jeonghkm@gnu.ac.kr $u Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, 52828, Korea. jeonghkm@gnu.ac.kr
773    0_
$w MED00011005 $t Folia microbiologica $x 1874-9356 $g Roč. 66, č. 5 (2021), s. 787-795
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34128186 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20211206 $b ABA008
991    __
$a 20211206103716 $b ABA008
999    __
$a ok $b bmc $g 1731478 $s 1150031
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 66 $c 5 $d 787-795 $e 20210614 $i 1874-9356 $m Folia microbiologica $n Folia microbiol. (Prague) $x MED00011005
GRA    __
$a 2017R1D1A1B03030037 $p Ministry of Education
LZP    __
$a Pubmed-20211206

Najít záznam

Citační ukazatele

Možnosti archivace