• Je něco špatně v tomto záznamu ?

Plant LHC-like proteins show robust folding and static non-photochemical quenching

P. Skotnicová, H. Staleva-Musto, V. Kuznetsova, D. Bína, MM. Konert, S. Lu, T. Polívka, R. Sobotka

. 2021 ; 12 (1) : 6890. [pub] 20211125

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22003168

Grantová podpora
854126 EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
19-28323X Grantová Agentura České Republiky (Grant Agency of the Czech Republic)

Life on Earth depends on photosynthesis, the conversion of light energy into chemical energy. Plants collect photons by light harvesting complexes (LHC)-abundant membrane proteins containing chlorophyll and xanthophyll molecules. LHC-like proteins are similar in their amino acid sequence to true LHC antennae, however, they rather serve a photoprotective function. Whether the LHC-like proteins bind pigments has remained unclear. Here, we characterize plant LHC-like proteins (LIL3 and ELIP2) produced in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). Both proteins were associated with chlorophyll a (Chl) and zeaxanthin and LIL3 was shown to be capable of quenching Chl fluorescence via direct energy transfer from the Chl Qy state to zeaxanthin S1 state. Interestingly, the ability of the ELIP2 protein to quench can be acquired by modifying its N-terminal sequence. By employing Synechocystis carotenoid mutants and site-directed mutagenesis we demonstrate that, although LIL3 does not need pigments for folding, pigments stabilize the LIL3 dimer.

Citace poskytuje Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22003168
003      
CZ-PrNML
005      
20220127150618.0
007      
ta
008      
220113s2021 xxk f 000 0|eng||
009      
AR
024    7_
$a 10.1038/s41467-021-27155-1 $2 doi
035    __
$a (PubMed)34824207
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a xxk
100    1_
$a Skotnicová, Petra $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, Czech Republic
245    10
$a Plant LHC-like proteins show robust folding and static non-photochemical quenching / $c P. Skotnicová, H. Staleva-Musto, V. Kuznetsova, D. Bína, MM. Konert, S. Lu, T. Polívka, R. Sobotka
520    9_
$a Life on Earth depends on photosynthesis, the conversion of light energy into chemical energy. Plants collect photons by light harvesting complexes (LHC)-abundant membrane proteins containing chlorophyll and xanthophyll molecules. LHC-like proteins are similar in their amino acid sequence to true LHC antennae, however, they rather serve a photoprotective function. Whether the LHC-like proteins bind pigments has remained unclear. Here, we characterize plant LHC-like proteins (LIL3 and ELIP2) produced in the cyanobacterium Synechocystis sp. PCC 6803 (hereafter Synechocystis). Both proteins were associated with chlorophyll a (Chl) and zeaxanthin and LIL3 was shown to be capable of quenching Chl fluorescence via direct energy transfer from the Chl Qy state to zeaxanthin S1 state. Interestingly, the ability of the ELIP2 protein to quench can be acquired by modifying its N-terminal sequence. By employing Synechocystis carotenoid mutants and site-directed mutagenesis we demonstrate that, although LIL3 does not need pigments for folding, pigments stabilize the LIL3 dimer.
650    _2
$a proteiny huseníčku $x chemie $x genetika $x metabolismus $7 D029681
650    _2
$a karotenoidy $x metabolismus $7 D002338
650    _2
$a chlorofyl $x metabolismus $7 D002734
650    _2
$a proteiny chloroplastové $x chemie $x genetika $x metabolismus $7 D060365
650    _2
$a přenos energie $7 D004735
650    _2
$a mutace $7 D009154
650    _2
$a vazba proteinů $7 D011485
650    _2
$a sbalování proteinů $7 D017510
650    _2
$a multimerizace proteinu $7 D055503
650    _2
$a Synechocystis $x genetika $x metabolismus $7 D046939
650    _2
$a xanthofyly $x metabolismus $7 D024341
650    _2
$a zeaxanthiny $x genetika $x metabolismus $7 D065146
655    _2
$a časopisecké články $7 D016428
655    _2
$a práce podpořená grantem $7 D013485
700    1_
$a Staleva-Musto, Hristina $u Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic
700    1_
$a Kuznetsova, Valentyna $u Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic
700    1_
$a Bína, David $u Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic $u Biology Centre, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic
700    1_
$a Konert, Minna M $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, Czech Republic
700    1_
$a Lu, Shan $u School of Life Sciences, Nanjing University, Nanjing, China
700    1_
$a Polívka, Tomáš $u Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic. tpolivka@jcu.cz $u Biology Centre, Institute of Plant Molecular Biology, Academy of Sciences of the Czech Republic, České Budějovice, Czech Republic. tpolivka@jcu.cz
700    1_
$a Sobotka, Roman $u Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, Czech Republic. sobotka@alga.cz
773    0_
$w MED00184850 $t Nature communications $x 2041-1723 $g Roč. 12, č. 1 (2021), s. 6890
856    41
$u https://pubmed.ncbi.nlm.nih.gov/34824207 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y p $z 0
990    __
$a 20220113 $b ABA008
991    __
$a 20220127150614 $b ABA008
999    __
$a ok $b bmc $g 1750817 $s 1154317
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2021 $b 12 $c 1 $d 6890 $e 20211125 $i 2041-1723 $m Nature communications $n Nat Commun $x MED00184850
GRA    __
$a 854126 $p EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
GRA    __
$a 19-28323X $p Grantová Agentura České Republiky (Grant Agency of the Czech Republic)
LZP    __
$a Pubmed-20220113

Najít záznam

Citační ukazatele

Možnosti archivace