Detail
Article
Online article
FT
Medvik - BMC
  • Something wrong with this record ?

Long-Term Cultures of Spinal Cord Interneurons

I. Vargova, J. Kriska, JCF. Kwok, JW. Fawcett, P. Jendelova

. 2022 ; 16 (-) : 827628. [pub] 20220207

Language English Country Switzerland

Document type Journal Article

Persistent link   https://www.medvik.cz/link/bmc22010064

Grant support
MR/V002694/1 Medical Research Council - United Kingdom

Spinal cord interneurons (SpINs) are highly diverse population of neurons that play a significant role in circuit reorganization and spontaneous recovery after spinal cord injury. Regeneration of SpIN axons across rodent spinal injuries has been demonstrated after modification of the environment and neurotrophin treatment, but development of methods to enhance the intrinsic regenerative ability of SpINs is needed. There is a lack of described in vitro models of spinal cord neurons in which to develop new regeneration treatments. For this reason, we developed a new model of mouse primary spinal cord neuronal culture in which to analyze maturation, morphology, physiology, connectivity and regeneration of identified interneurons. Isolated from E14 mice, the neurons mature over 15 days in vitro, demonstrated by expression of maturity markers, electrophysiological patch-clamp recordings, and formation of synapses. The neurons express markers of SpINs, including Tlx3, Lmx1b, Lbx1, Chx10, and Pax2. The neurons demonstrate distinct morphologies and some form perineuronal nets in long-term cultivation. Live neurons in various maturation stages were axotomized, using a 900 nm multiphoton laser and their fate was observed overnight. The percentage of axons that regenerated declined with neuronal maturity. This model of SpINs will be a valuable tool in future regenerative, developmental, and functional studies alongside existing models using cortical or hippocampal neurons.

References provided by Crossref.org

000      
00000naa a2200000 a 4500
001      
bmc22010064
003      
CZ-PrNML
005      
20220425131658.0
007      
ta
008      
220420s2022 sz f 000 0|eng||
009      
AR
024    7_
$a 10.3389/fncel.2022.827628 $2 doi
035    __
$a (PubMed)35197829
040    __
$a ABA008 $b cze $d ABA008 $e AACR2
041    0_
$a eng
044    __
$a sz
100    1_
$a Vargova, Ingrid $u Department of Neuroregeneration, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czechia $u Second Faculty of Medicine, Charles University, Prague, Czechia
245    10
$a Long-Term Cultures of Spinal Cord Interneurons / $c I. Vargova, J. Kriska, JCF. Kwok, JW. Fawcett, P. Jendelova
520    9_
$a Spinal cord interneurons (SpINs) are highly diverse population of neurons that play a significant role in circuit reorganization and spontaneous recovery after spinal cord injury. Regeneration of SpIN axons across rodent spinal injuries has been demonstrated after modification of the environment and neurotrophin treatment, but development of methods to enhance the intrinsic regenerative ability of SpINs is needed. There is a lack of described in vitro models of spinal cord neurons in which to develop new regeneration treatments. For this reason, we developed a new model of mouse primary spinal cord neuronal culture in which to analyze maturation, morphology, physiology, connectivity and regeneration of identified interneurons. Isolated from E14 mice, the neurons mature over 15 days in vitro, demonstrated by expression of maturity markers, electrophysiological patch-clamp recordings, and formation of synapses. The neurons express markers of SpINs, including Tlx3, Lmx1b, Lbx1, Chx10, and Pax2. The neurons demonstrate distinct morphologies and some form perineuronal nets in long-term cultivation. Live neurons in various maturation stages were axotomized, using a 900 nm multiphoton laser and their fate was observed overnight. The percentage of axons that regenerated declined with neuronal maturity. This model of SpINs will be a valuable tool in future regenerative, developmental, and functional studies alongside existing models using cortical or hippocampal neurons.
655    _2
$a časopisecké články $7 D016428
700    1_
$a Kriska, Jan $u Department of Cellular Neurophysiology, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czechia
700    1_
$a Kwok, Jessica C F $u The Center for Reconstructive Neuroscience, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czechia $u Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom
700    1_
$a Fawcett, James W $u The Center for Reconstructive Neuroscience, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czechia $u John van Geest Centre for Brain Repair, Department of Clinical Neurosciences, University of Cambridge, Cambridge, United Kingdom
700    1_
$a Jendelova, Pavla $u Department of Neuroregeneration, Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czechia $u Second Faculty of Medicine, Charles University, Prague, Czechia
773    0_
$w MED00163312 $t Frontiers in cellular neuroscience $x 1662-5102 $g Roč. 16, č. - (2022), s. 827628
856    41
$u https://pubmed.ncbi.nlm.nih.gov/35197829 $y Pubmed
910    __
$a ABA008 $b sig $c sign $y - $z 0
990    __
$a 20220420 $b ABA008
991    __
$a 20220425131655 $b ABA008
999    __
$a ind $b bmc $g 1784475 $s 1161262
BAS    __
$a 3
BAS    __
$a PreBMC
BMC    __
$a 2022 $b 16 $c - $d 827628 $e 20220207 $i 1662-5102 $m Frontiers in cellular neuroscience $n Front Cell Neurosci $x MED00163312
GRA    __
$a MR/V002694/1 $p Medical Research Council $2 United Kingdom
LZP    __
$a Pubmed-20220420

Find record

Citation metrics

Archiving options