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Non-invasive method of obtaining milk from mice using carbetocin, a synthetic analogue of oxytocin
P. Klein, I. Kleinova, V. Rucka, J. Nevoral
Jazyk angličtina Země Spojené státy americké
Typ dokumentu časopisecké články
- MeSH
- mléko * MeSH
- myši inbrední ICR MeSH
- myši MeSH
- oxytocin * analogy a deriváty farmakologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
INTRODUCTION: The mouse is an important laboratory animal in pharmacological and toxicological research. However, there is a limited ability to analyse the penetration of tested substances in mouse breastmilk, which is technically difficult to obtain in sufficient quantities. The aim of the study is to verify the use of carbetocin for this purpose. METHODS: The effect of carbetocin (20 and 40 μg per animal) was tested in nursing ICR females that had milk collected for 15 min, started 1.5 min after administration. At a higher dose, carbetocin was also tested with a 20-min collection, started 7 min after application. Oxytocin (2 IU per animal) and saline were used as positive and negative controls, respectively. RESULTS: Milk yield using a lower dose of carbetocin was comparable to oxytocin. However, the duration of action of carbetocin was longer than that of oxytocin. A higher doses of carbetocin resulted in significantly higher milk volumes. DISCUSSION: The use of carbetocin has proven to be an effective non-invasive method to obtain up to 0.89 g of milk from one mouse in 20 min.
Citace poskytuje Crossref.org
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- $a INTRODUCTION: The mouse is an important laboratory animal in pharmacological and toxicological research. However, there is a limited ability to analyse the penetration of tested substances in mouse breastmilk, which is technically difficult to obtain in sufficient quantities. The aim of the study is to verify the use of carbetocin for this purpose. METHODS: The effect of carbetocin (20 and 40 μg per animal) was tested in nursing ICR females that had milk collected for 15 min, started 1.5 min after administration. At a higher dose, carbetocin was also tested with a 20-min collection, started 7 min after application. Oxytocin (2 IU per animal) and saline were used as positive and negative controls, respectively. RESULTS: Milk yield using a lower dose of carbetocin was comparable to oxytocin. However, the duration of action of carbetocin was longer than that of oxytocin. A higher doses of carbetocin resulted in significantly higher milk volumes. DISCUSSION: The use of carbetocin has proven to be an effective non-invasive method to obtain up to 0.89 g of milk from one mouse in 20 min.
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