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Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

D. Rodriguez-Temporal, F. Alcaide, I. Mareković, JA. O'Connor, R. Gorton, J. van Ingen, A. Van den Bossche, G. Héry-Arnaud, C. Beauruelle, D. Orth-Höller, JJ. Palacios-Gutiérrez, G. Tudó, G. Bou, PJ. Ceyssens, M. Garrigó, J. González-Martin, G....

. 2022 ; 12 (1) : 1237. [pub] 20220124

Jazyk angličtina Země Velká Británie

Typ dokumentu časopisecké články, multicentrická studie, práce podpořená grantem

Perzistentní odkaz   https://www.medvik.cz/link/bmc22011276

Grantová podpora
PI18/01068 Health Research Fund (Instituto de Salud Carlos III. Plan Nacional de I+D+I 2013-2016)
PI18/00997 Health Research Fund (Instituto de Salud Carlos III. Plan Nacional de I+D+I 2013-2016)
PI15/01073 Instituto de Salud Carlos III

The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2-98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.

Biomedical Center Faculty of Medicine in Plzen Plzen Czech Republic

Department of Clinical and Molecular Microbiology University Hospital Centre Zagreb Zagreb Croatia

Department of Clinical Microbiology and Infectious Diseases Hospital General Universitario Gregorio Marañón Madrid Spain

Department of Medical Microbiology MVZ Dr Eberhard and Partner Dortmund Dortmund Germany

Dept of Biological Sciences Munster Technological University Cork Ireland

Division of Human Bacterial diseases Sciensano Brussels Belgium

Division of Hygiene and Medical Microbiology Medical University of Innsbruck Innsbruck Austria

Health Services Laboratories London UK

Institut de Microbiologie Université de Lausanne Lausanne Switzerland

Laboratorie de Bactériologie Plateforme de Biologie Hôpital Cavale Blanche Brest France

Laboratorio de Microbiologia Hospital de la Santa Creu i Sant Pau Barcelona Spain

Laboratorio de Microbiología Hospital Regional de Málaga Málaga Spain

Oslo University Hospital Oslo Norway

Radboud University Medical Centre Nijmegen The Netherlands

Servei de Microbiologia CDB Hospital Clínic de Barcelona ISGlobal Departament Fonaments Clínics Facultat de Medicina i Ciències de la Salut Universitat de Barcelona Barcelona Spain

Servei de Microbiologia Hospital Universitari de Bellvitge IDIBELL Departament de Patologia i Terapèutica Experimental Facultat de Medicina i Ciències de la Salut Universitat de Barcelona Hospitalet de Llobregat Spain

Servicio de Microbiología Hospital Universitario A Coruña Coruña Spain

Servicio de Microbiología Hospital Universitario Central de Asturias Oviedo Spain

Citace poskytuje Crossref.org

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